Zhang Yi, Gong Wenfeng, Gao Yingying, Zhao Kun, Wang Fangzhan, Liu Yapei, Zhang Mei, Yu Xiaolin
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Laboratory of Horticultural Plant Growth & Quality Regulation, Ministry of Agriculture, Hangzhou 310058, China.
Plant Dis. 2024 Dec;108(12):3473-3483. doi: 10.1094/PDIS-12-23-2748-SR. Epub 2024 Nov 18.
Clubroot, caused by , is a globally destructive soilborne disease affecting cruciferous plants. In this study, the predominant pathotypes of . in six cities within Zhejiang Province were identified using the Williams and European clubroot differential (ECD) systems. A phylogenetic analysis of . isolates infecting cruciferous crops worldwide was conducted using MEGA, and their ITS2 secondary structures were predicted through the ITS2 database. Accessions of , . , . , and Mill. were employed to assess clubroot resistance. The results revealed that the prevalent pathotypes in Zhejiang Province were pathotype 1, ECD20/31/12 and ECD24/16/30; pathotype 3, ECD20/15/4; pathotype 8, ECD16/0/0 and ECD24/0/0; and pathotype 2, ECD16/15/15. Isolates from distinct genera of Brassicaceae formed separate branches in the evolutionary tree. Moreover, isolates of crops from Zhejiang Province exhibited homology with those from other global regions, a finding corroborated by their ITS2 secondary structure. Approximately 80 and 95% of . and . crops displayed susceptible phenotypes for pathotype 8, ECD16/0/0, whereas approximately 60% of . crops exhibited resistance. Furthermore, three crop accessions showed significant variation in resistance to the pathogen, both among morphological and geographical origin groups. This study contributes to understanding the distribution of diverse . pathotypes in different regions of Zhejiang Province and facilitates the identification of crops with potential disease resistance suitable for cultivation in the province.
根肿病由[病原体名称未给出]引起，是一种对全球十字花科植物具有毁灭性的土传病害。在本研究中，使用威廉姆斯和欧洲根肿病鉴别（ECD）系统鉴定了浙江省六个城市中[病原体名称未给出]的主要致病型。利用MEGA对全球感染十字花科作物的[病原体名称未给出]分离株进行了系统发育分析，并通过ITS2数据库预测了它们的ITS2二级结构。使用[植物名称未给出]、[植物名称未给出]、[植物名称未给出]和[植物名称未给出]的种质来评估对根肿病的抗性。结果表明，浙江省流行的致病型为致病型1，ECD20/31/12和ECD24/16/30；致病型3，ECD20/15/4；致病型8，ECD16/0/0和ECD24/0/0；以及致病型2，ECD16/15/15。十字花科不同属的分离株在进化树中形成了单独的分支。此外，浙江省[作物名称未给出]作物的分离株与全球其他地区的分离株表现出同源性，这一发现通过它们的ITS2二级结构得到了证实。大约80%和95%的[作物名称未给出]和[作物名称未给出]作物对致病型8，ECD16/0/0表现出易感表型，而大约60%的[作物名称未给出]作物表现出抗性。此外，三种[作物名称未给出]作物种质在对病原体的抗性方面，在形态和地理起源组之间均表现出显著差异。本研究有助于了解浙江省不同地区多种[病原体名称未给出]致病型的分布，并有助于鉴定适合在该省种植的具有潜在抗病性的[作物名称未给出]作物。
