Huangwei Zhang, Peiyuan Jin, Yixuan Kong, Zhimin Yang, Yuxin Zhou, Geunhwa Jung, Jian Hu
College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
Front Microbiol. 2023 Sep 29;14:1266045. doi: 10.3389/fmicb.2023.1266045. eCollection 2023.
Emerging multidrug resistance (MDR) in spp. is a huge challenge to the management of dollar spot (DS) disease on turfgrass. Insight into the molecular basis of resistance mechanisms may help identify key molecular targets for developing novel effective chemicals. Previously, a MDR isolate (LT586) of with significantly reduced sensitivities to propiconazole, boscalid, and iprodione, and a fungicide-sensitive isolate (LT15) of the same species were isolated from creeping bentgrass ( L.). The present study aimed to further explore the molecular mechanisms of resistance by using genome-wide transcriptional analyses of the two isolates. A total of 619 and 475 differentially expressed genes (DEGs) were significantly down and upregulated in the MDR isolate LT586, compared with the sensitive isolate LT15 without fungicide treatment. Three hundreds and six and 153 DEGs showed significantly lower and higher expression in the MDR isolate LT586 than those in the sensitive isolate LT15, which were commonly induced by the three fungicides. Most of the 153 upregulated DEGs were xenobiotic detoxification-related genes and genes with transcriptional functions. Fifty and 17 upregulated DEGs were also commonly observed in HRI11 (a MDR isolate of the ) compared with the HRS10 (a fungicide-sensitive isolate of same species) from a previous study without and with the treatment of propiconazole, respectively. The reliability of RNA-seq data was further verified by qRT-PCR method using a few select potentially MDR-related genes. Results of this study indicated that there were multiple uncharacterized genes, possibly responsible for MDR phenotypes in spp., which may have important implications in understanding the molecular mechanisms underlying MDR resistance.
病原菌中出现的多重耐药性（MDR）对草坪草美元斑病（DS）的防治构成了巨大挑战。深入了解耐药机制的分子基础可能有助于确定开发新型有效化学药剂的关键分子靶点。此前，从匍匐翦股颖（Agrostis stolonifera L.）中分离出了对丙环唑、啶酰菌胺和异菌脲敏感性显著降低的病原菌多重耐药分离株（LT586）以及同一物种的杀菌剂敏感分离株（LT15）。本研究旨在通过对这两个分离株进行全基因组转录分析，进一步探究耐药的分子机制。与未用杀菌剂处理的敏感分离株LT15相比，多重耐药分离株LT586中共有619个和475个差异表达基因（DEG）显著下调和上调。306个和153个DEG在多重耐药分离株LT586中的表达显著低于和高于敏感分离株LT15，这些基因通常由三种杀菌剂诱导。153个上调的DEG中大多数是与异源生物解毒相关的基因和具有转录功能的基因。与之前分别未处理和用丙环唑处理的研究中的敏感分离株HRS10相比，在HRI11（病原菌的多重耐药分离株）中也分别普遍观察到50个和17个上调的DEG。通过对一些选定的潜在多重耐药相关基因采用qRT-PCR方法进一步验证了RNA测序数据的可靠性。本研究结果表明，存在多个未表征的基因，可能与病原菌的多重耐药表型有关，这可能对理解多重耐药抗性的分子机制具有重要意义。
