Ivanova-Pozdejeva Anna, Jakobson Liina, Ilves Kai, Kivistik Agnes, Kann Liina, Aida Jekaterina, Kübarsepp Liisa, Tähtjärv Terje, Laanemets Kristiina
Department of Plant Biotechnology, Centre of Estonian Rural Research and Knowledge (METK), M. Pilli haru 1, Jõgeva 48309, Estonia.
Department of Plant Protection, Centre of Estonian Rural Research and Knowledge (METK), M. Pilli haru 1, Jõgeva 48309, Estonia.
Breed Sci. 2023 Jun;73(3):300-312. doi: 10.1270/jsbbs.22094. Epub 2023 Jul 7.
resistance has been an important trait in potato () breeding for decades. Our aim was to complement phenotypic testing with genetic marker analysis. We analysed the results of resistance greenhouse testing in 4601 tubers of 2918 breeding clones from 11 years. Applicability of gene markers TG689 and 57R was compared. We implemented the latter with the positive predictive value of 99.1% and negative predictive value of 60.0% into the breeding scheme. The 57R marker alleles of 22 Estonian cultivars and 470 breeding clones were determined. Two unique 57R alleles, 57R-887 and 57R-1155, were found in Estonian cultivar 'Anti'. The 887 bp allele has two deletions (14 bp and 490 bp) accompanied by several other indels and SNPs within the 57R marker region. The 1155 bp allele has three deletions (7 bp, 20 bp and 210 bp) accompanied by several other indels and SNPs within the same region. Partial resistance to in 'Anti' suggests that the newly described alleles could affect the -mediated resistance directly or indirectly.
几十年来，抗性一直是马铃薯育种中的一个重要性状。我们的目标是用基因标记分析来补充表型测试。我们分析了来自11年的2918个育种克隆的4601个块茎的抗性温室测试结果。比较了基因标记TG689和57R的适用性。我们将后者以99.1%的阳性预测值和60.0%的阴性预测值纳入育种方案。确定了22个爱沙尼亚品种和470个育种克隆的57R标记等位基因。在爱沙尼亚品种“Anti”中发现了两个独特的57R等位基因，57R-887和57R-1155。887 bp等位基因有两个缺失（14 bp和490 bp），同时在57R标记区域内还有其他几个插入缺失和单核苷酸多态性。1155 bp等位基因有三个缺失（7 bp、20 bp和210 bp），在同一区域内还有其他几个插入缺失和单核苷酸多态性。“Anti”对的部分抗性表明，新描述的等位基因可能直接或间接影响介导的抗性。
