Mei Hongjun, Tan Junfeng, Hu You, Shi Xiangwei, Liu Yang, Jia Fan, Xu Fuqiang
Department of Orthopaedics, The Fifth hospital of Wuhan, Wuhan 430050, China.
Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.
IBRO Neurosci Rep. 2023 Oct 2;15:235-241. doi: 10.1016/j.ibneur.2023.09.011. eCollection 2023 Dec.
Nerve growth factor (NGF) has been shown to support the survival and differentiation of neurons. In this study, we first developed a retrograde trans-multisynaptic tracer PRV580 expressing the mCherry fluorescent protein based on pseudorabies virus Bartha strain to map the neural circuit of sciatic nerve. Secondly, the newly developed PRV580 was used to map the neural circuit of the recovering sciatic nerve upon treatment with NGF. Our results showed that red signals from PRV580 were observed in various brain regions. Among these regions, many areas of the pyramidal system and the extra-pyramidal system had been mapped, accounting for as much as 56.8 % of the total inputs. Furthermore, we found that NGF could significantly increase the ratio of total input (29.05 %) compared to PBS (3.65 %), indicating that NGF indeed can aid in the repair of injured sciatic nerve. These findings indicated that NGF has therapeutic ability for the treatment of peripheral nerve injuries and virus-based tracers can be used to monitor the recovery.
神经生长因子（NGF）已被证明能支持神经元的存活和分化。在本研究中，我们首先基于伪狂犬病病毒巴塔株开发了一种表达mCherry荧光蛋白的逆行跨多突触示踪剂PRV580，用于绘制坐骨神经的神经回路。其次，使用新开发的PRV580来绘制经NGF治疗后坐骨神经恢复的神经回路。我们的结果显示，在各个脑区都观察到了PRV580发出的红色信号。在这些区域中，锥体系统和锥体外系统的许多区域都已被绘制出来，占总输入的56.8%。此外，我们发现与PBS（3.65%）相比，NGF能显著提高总输入的比例（29.05%），这表明NGF确实有助于受损坐骨神经的修复。这些发现表明，NGF对周围神经损伤具有治疗能力，基于病毒的示踪剂可用于监测恢复情况。
