基于组成噬菌体的生理特性定制用于长期裂解的有效噬菌体鸡尾酒。
Tailoring Effective Phage Cocktails for Long-Term Lysis of Based on Physiological Properties of Constituent Phages.
作者信息
Kaneko Tomoyoshi, Osaka Toshifumi, Tsuneda Satoshi
机构信息
Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.
Phage Therapy Institute, Comprehensive Research Organization, Waseda University, Tokyo, Japan.
出版信息
Phage (New Rochelle). 2023 Sep 1;4(3):128-135. doi: 10.1089/phage.2023.0016. Epub 2023 Sep 20.
BACKGROUND
Bacteriophage (phage) therapy has regained attention as an alternative to antimicrobial agents for eliminating bacteria; however, the emergence of phage-resistant bacteria during the therapy is a major concern. One method to control this emergence is to create a cocktail composed of multiple phages.
MATERIALS AND METHODS
In this study, we isolated 28 phages infecting and evaluated their bacteriolysis (lysis) activity, lytic spectrum, adsorption rate constant, burst size, and titer of a 1-day incubation, followed by clustering of the phages based on these physiological characteristics.
RESULTS
The variation in lysis onset time and duration was more significant for cocktails of phages from different clusters than for phage cocktails from the same cluster.
CONCLUSIONS
This suggests that a combination of phages with different physiological characteristics is necessary to create a cocktail that rapidly and continuously lyses bacteria over a prolonged duration while suppressing the emergence of resistant bacterial strains.
背景
噬菌体疗法作为消除细菌的抗菌剂替代品已重新受到关注；然而，治疗期间噬菌体抗性细菌的出现是一个主要问题。控制这种出现的一种方法是创建由多种噬菌体组成的混合物。
材料与方法
在本研究中，我们分离出28种感染[细菌名称未给出]的噬菌体，并评估了它们的细菌溶解（裂解）活性、裂解谱、吸附速率常数、爆发量和1天培养后的滴度，然后根据这些生理特征对噬菌体进行聚类。
结果
来自不同簇的噬菌体混合物的裂解起始时间和持续时间的变化比来自同一簇的噬菌体混合物更显著。
结论
这表明，有必要将具有不同生理特征的噬菌体组合起来，以创建一种混合物，在长时间内快速、持续地裂解细菌，同时抑制抗性菌株的出现。
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