Faculty of pharmacy and biochemistry, University of Zagreb, Zagreb, Croatia.
Department of laboratory diagnostics, University hospital centre Zagreb, Zagreb, Croatia.
Biochem Med (Zagreb). 2023 Oct 15;33(3):030702. doi: 10.11613/BM.2023.030702.
Olanzapine is an atypical antipsychotic drug which is effective in the treatment of schizophrenia. Cigarette smoking, age, and sex could be related to the pharmacokinetics and serum concentrations of olanzapine in patients with schizophrenia. The aim of the study was to examine whether there was a significant difference in the serum olanzapine concentrations with regard to the mentioned factors.
A total of 58 outpatients with schizophrenia (37 smokers, 42 men, 35 older than 40 years) participated in the study. Blood was sampled in serum tubes just before taking the next dose of olanzapine. Olanzapine was extracted by liquid-liquid extraction and was measured by an in-house high-performance liquid chromatography method on Shimadzu Prominence HPLC System with diode array detector SPD-M20A (Shimadzu, Kyoto, Japan). The results were expressed as the ratio of concentration to the daily dose of olanzapine (C/D). Non-parametric statistical tests were used to analyse differences between variables.
The median C/D of olanzapine (interquartile range) in smokers was 6.0 (3.4-10.2) nmol/L/mg and in non-smokers 10.1 (5.9-17.6) nmol/L/mg; P = 0.007. The median C/D of olanzapine in patients younger than 40 years was 5.6 (4.5-10.2) nmol/L/mg and in patients older than 40 years 8.4 (5.6-13.0) nmol/L/mg; P = 0.105. The median C/D of olanzapine in male patients was 6.6 (4.6-10.4) nmol/L/mg and in female patients 9.0 (5.9-15.3) nmol/L/mg; P = 0.064.
The serum olanzapine concentration was significantly lower in smoking than in non-smoking patients with schizophrenia. No significant difference was demonstrated with regard to age and sex.
奥氮平是一种新型抗精神病药物，对精神分裂症的治疗有效。吸烟、年龄和性别可能与精神分裂症患者奥氮平的药代动力学和血清浓度有关。本研究旨在研究血清奥氮平浓度是否因上述因素而存在显著差异。
共有 58 名精神分裂症门诊患者（37 名吸烟者、42 名男性、35 名年龄大于 40 岁）参与了这项研究。在服用奥氮平下一剂之前，采集血清管中的血液。采用液液萃取法提取奥氮平，用 Shimadzu Prominence HPLC 系统（日本京都岛津公司）与二极管阵列检测器 SPD-M20A 进行内部高效液相色谱法测定。结果表示为浓度与奥氮平日剂量的比值（C/D）。采用非参数统计检验分析变量之间的差异。
吸烟者奥氮平的 C/D 中位数（四分位距）为 6.0（3.4-10.2）nmol/L/mg，非吸烟者为 10.1（5.9-17.6）nmol/L/mg；P=0.007。年龄小于 40 岁的患者奥氮平的 C/D 中位数为 5.6（4.5-10.2）nmol/L/mg，年龄大于 40 岁的患者为 8.4（5.6-13.0）nmol/L/mg；P=0.105。男性患者奥氮平的 C/D 中位数为 6.6（4.6-10.4）nmol/L/mg，女性患者为 9.0（5.9-15.3）nmol/L/mg；P=0.064。
吸烟精神分裂症患者的血清奥氮平浓度明显低于非吸烟患者。年龄和性别方面无显著差异。
