CAS Key Laboratory of Agroecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China.
College of Advanced Agricultural, University of Chinese Academy of Sciences, Beijing, China.
Front Cell Infect Microbiol. 2023 Sep 29;13:1264550. doi: 10.3389/fcimb.2023.1264550. eCollection 2023.
As a potential antibiotic alternative, macleaya cordata extract (MCE) has anti-inflammatory, antioxidant, and antimicrobial properties. This study was conducted to assess the impact of MCE supplementation on the gut microbiota and its interplay with the host in young goats. Thirty female black goats with similar body weight (5.63 ± 0.30 kg) were selected and randomly allotted into one of three diets: a control diet (Control), a control diet with antibiotics (Antibiotics, 21 mg/kg/day vancomycin and 42 mg/kg/day neomycin), and a control diet with MCE (MCE, 3.75% w/w premix).
Principal coordinate analysis of the microbial community showed that samples of Antibiotic clustered separately from both Control and MCE ( < 0.001). The random forest analysis revealed that, in comparison to the Control group, the impact of Antibiotics on the microbiota structure was more pronounced than that of MCE (number of featured microbiota, 13 in Antibiotics and >6 in MCE). In addition, the pathways of significant enrichment either from DEGs between Antibiotics and Control or from DEGs between MCE and Control were almost identical, including Th17 cell differentiation, butanoate metabolism, T-cell receptor signaling pathway, intestinal immune network for IgA production, antigen processing and presentation, and ABC transporters. Furthermore, an integrative analysis indicated that significant positive correlations ( < 0.05) were observed between and the featured biomarkers , , , and in the MCE group. Conversely, several significant negative correlations ( < 0.05) were identified between and the featured biomarkers , , , , , , , , , and in the Antibiotics group.
Collectively, the analysis of microbiome-transcriptome data revealed that dietary supplementation with MCE produced significant alterations in multiple immune pathways, while having minimal impact on the microbial structure.
作为一种有潜力的抗生素替代品，博落回提取物（MCE）具有抗炎、抗氧化和抗菌特性。本研究旨在评估 MCE 补充对幼山羊肠道微生物群及其与宿主相互作用的影响。选择 30 只体重相似（5.63±0.30kg）的雌性黑山羊，随机分为三组：对照组（Control）、抗生素对照组（Antibiotics，21mg/kg/天万古霉素和 42mg/kg/天新霉素）和 MCE 对照组（MCE，3.75%w/w 预混料）。
微生物群落的主坐标分析表明，Antibiotics 组的样本与 Control 和 MCE 组均明显分离（ < 0.001）。随机森林分析显示，与 Control 组相比，Antibiotics 对微生物群落结构的影响比 MCE 更为显著（Antibiotics 组有 13 个特征菌群，而 MCE 组有 >6 个）。此外，Antibiotics 与 Control 组之间差异表达基因（DEGs）或 MCE 与 Control 组之间 DEGs 显著富集的通路几乎相同，包括 Th17 细胞分化、丁酸盐代谢、T 细胞受体信号通路、肠道免疫网络 IgA 产生、抗原加工和呈递以及 ABC 转运蛋白。此外，整合分析表明，在 MCE 组中，显著正相关（ < 0.05）存在于 和特征生物标志物 、 、 、 之间。相反，在 Antibiotics 组中， 与特征生物标志物 、 、 、 、 、 、 、 之间存在多个显著负相关（ < 0.05）。
综上所述，微生物组-转录组数据分析表明，MCE 膳食补充对多种免疫途径产生了显著影响，而对微生物结构的影响较小。
