Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, 32962, USA.
Independent researcher, Vero Beach, FL, 32962, USA.
F1000Res. 2023 Mar 24;12:330. doi: 10.12688/f1000research.130734.1. eCollection 2023.
Despite efforts to minimize the impacts of malaria and reduce the number of primary vectors, malaria has yet to be eliminated in Zambia. Understudied vector species may perpetuate malaria transmission in pre-elimination settings. is one of the most abundantly caught mosquito species in southern Zambia and has previously been found with sporozoites, a causal agent of human malaria. This species may be a critical vector of malaria transmission, however, there is a lack of genetic information available for We report the first genome data and the first complete mitogenome (Mt) sequence of The sequence was extracted from one individual mosquito from the Chidakwa area in Macha, Zambia. The raw reads were obtained using Illumina Novaseq 6000 and assembled through NOVOplasty alignment with related species. The length of the Mt was 15,351 bp, with 77.9 % AT content. The closest match to the whole mitochondrial genome in the phylogenetic tree is the African malaria mosquito, Its genome data is available through National Center for Biotechnology Information (NCBI) Sequencing Reads Archive (SRA) with accession number SRR22114392. The mitochondrial genome was deposited in NCBI GenBank with the accession number OP776919. The ITS2 containing contig sequence was deposited in GenBank with the accession number OQ241725. Mitogenome annotation and a phylogenetic tree with related mosquito species are provided.
尽管已经努力最小化疟疾的影响并减少主要传播媒介的数量,但赞比亚仍未能消灭疟疾。研究不足的媒介物种可能会在消除前环境中继续传播疟疾。 是赞比亚南部最常捕获的蚊子物种之一,以前曾在该物种中发现有疟原虫孢子,这是人类疟疾的病原体。该物种可能是疟疾传播的关键媒介,但是,对于 我们缺乏可用的遗传信息。我们报告了 的第一个基因组数据和第一个完整的线粒体基因组(Mt)序列。该序列是从赞比亚马查地区 Chidakwa 地区的一只蚊子中提取的。原始读数是使用 Illumina Novaseq 6000 获得的,并通过 NOVOplasty 与相关物种的比对进行组装。Mt 的长度为 15351 bp,具有 77.9%的 AT 含量。在系统发育树中与整个线粒体基因组最接近的是非洲疟蚊, 其基因组数据可通过国家生物技术信息中心(NCBI)测序读取档案(SRA)获得,登录号为 SRR22114392。线粒体基因组已在 NCBI GenBank 中以登录号 OP776919 进行了存放。包含 ITS2 的拼接序列已在 GenBank 中以登录号 OQ241725 进行了存放。提供了线粒体基因组注释和与相关 蚊子物种的系统发育树。