The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Tropical Diseases Research Centre, Ndola, Zambia.
F1000Res. 2024 May 30;13:553. doi: 10.12688/f1000research.148473.1. eCollection 2024.
Mosquitoes belonging to the genus are the only vectors of human malaria. has been linked to malaria transmission in Kenya, with recent collections in Zambia reporting the mosquito species exhibiting zoophilic and exophilic behavioral patterns with occasional contact with humans. Given the paucity of genetic data, and challenges to identification and molecular taxonomy of the mosquitoes belonging to the genus; we report the first complete mitochondrial genome of using a genome skimming approach. An Illumina Novaseq 6000 platform was used for sequencing, the length of the mitochondrial genome was 15401 bp, with 78.5% AT content comprised of 37 genes. Phylogenetic analysis by maximum likelihood using concatenation of the 13 protein coding genes demonstrated that was the closest relative based on existing sequence data. This study demonstrates that the skimming approach is an inexpensive and efficient approach for mosquito species identification and concurrent taxonomic rectification, which may be a useful alternative for generating reference sequence data for evolutionary studies among the Culicidae.
属于属的蚊子是人类疟疾的唯一传播媒介。已与肯尼亚的疟疾传播有关，最近在赞比亚的采集报告显示，该蚊种表现出嗜动物和嗜外生性行为模式，偶尔与人类接触。鉴于遗传数据的缺乏，以及对属于属的蚊子进行鉴定和分子分类学的挑战；我们使用基因组掠过法报告了使用基因组掠过法的的第一个完整线粒体基因组。Illumina Novaseq 6000 平台用于测序，线粒体基因组的长度为 15401bp，具有 78.5%的 AT 含量，由 37 个基因组成。使用 13 个蛋白质编码基因的串联进行最大似然系统发育分析表明，根据现有序列数据，是最接近的亲缘关系。这项研究表明，掠过法是一种廉价有效的蚊子种鉴定和同时分类纠正方法，对于在蚊科中进行进化研究生成参考序列数据可能是一种有用的替代方法。
