Bershadsky A D, Gelfand V I, Svitkina T M, Tint I S
Cell Biol Int Rep. 1979 Jan;3(1):45-50. doi: 10.1016/0309-1651(79)90067-5.
Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 degrees C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 degrees C.
在0℃长时间孵育后，用曲拉通X-100处理培养的小鼠胚胎成纤维细胞，可在冷却细胞的细胞质中发现微管网络。通过使用抗微管蛋白的单特异性抗体进行免疫荧光显微镜检查以及电子显微镜检查，证实了这种冷稳定微管网络的存在。冷稳定微管以及普通的细胞质微管对钙离子敏感，在用秋水仙碱或长春花碱预处理的细胞中未观察到。冷稳定微管在0℃时似乎与解聚的微管蛋白池不平衡。
