棉花中海藻糖-1,6-二磷酸酶样蛋白冷诱导释放的定量及其与脱落酸的关系
Quantitation of Chill-Induced Release of a Tubulin-Like Factor and Its Prevention by Abscisic Acid in Gossypium hirsutum L.
机构信息
Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel.
出版信息
Plant Physiol. 1983 Apr;71(4):747-8. doi: 10.1104/pp.71.4.747.
The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted in complete release of the tubulin-like factor. Pretreatment with abscisic acid in the light almost completely prevented the chill-induced release of the tubulin-like factor. Addition of colchicine during the chilling period accelerated the release of the tubulin-like factor. Pretreatment with abscisic acid greatly reduced this effect of colchicine. It is concluded that the destruction of the microtubular network is involved in the development of chilling injury in cotton. Abscisic acid apparently decreased chilling injury by stabilization of the microtubular network.
棉（Gossypium hirsutum L. cv Acala）子叶组织中微管蛋白聚合程度通过放射免疫测定法进行估计，该方法测量了一种微管蛋白样因子的含量。据推测，这种微管蛋白样因子的释放表明微管的解聚。冷胁迫导致微管蛋白样因子完全释放。在光照下用脱落酸预处理几乎完全阻止了冷胁迫引起的微管蛋白样因子的释放。在冷胁迫期间添加秋水仙素加速了微管蛋白样因子的释放。脱落酸预处理大大降低了秋水仙素的这种作用。因此可以得出结论，微管网络的破坏参与了棉花冷胁迫伤害的发展。脱落酸通过稳定微管网络明显减少了冷胁迫伤害。
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