伊朗霍拉马巴德医院产 ESBLs 肺炎克雷伯菌毒力因子的流行情况。

The Prevalence of Virulence Factors among ESBLs-Producing Klebsiella pneumoniae Isolated from Khorramabad Hospitals, Iran.

出版信息

Clin Lab. 2023 Oct 1;69(10). doi: 10.7754/Clin.Lab.2023.230322.

DOI:10.7754/Clin.Lab.2023.230322

Abstract

BACKGROUND

Klebsiella pneumoniae is one of the opportunistic bacteria that cause hospital infections. Various virulence factors are involved in its pathogenesis. The purpose of our study was to investigate the prevalence of virulence factor genes entB, mrkD, magA, kfu, iutA, rmpA, ybtS, and k2 among ESBLs-producing Klebsiella pneumoniae isolated from clinical samples of Khorramabad hospitals in Iran.

METHODS

In this descriptive, analytical study, the extended-spectrum β-lactamases (ESBLs) screening phenotypic test was performed by disc diffusion method, and virulence factor genes were detected by PCR method for Klebsiella pneumoniae isolates. Finally, the obtained data were statistically analyzed by SPSS software (version 21) using the chi-squared test.

RESULTS

One hundred and ten K. pneumoniae strains were isolated from urine 69 (62.7%), sputum 16 (14.5%), blood 10 (9.1%), wound 9 (8.2%), tissue 3 (2.7%), body fluids 2 (1.8%), and catheter 1(0.9%). Based on an ESBL screening phenotypic test, 57 (51.8%) K. pneumoniae isolates were ESBLs-producing. Among 57 ESBLs-positive K. pneumoniae, the occurrence of mrkD, entB, ybtS, iutA, kfu, k2, and rmpA genes were 88 (80%), 87 (79.1%), 65 (59.1%), 42 (38.2%), 19 (17.3%), 5 (4.5%), 3 (2.7%), and 2 (1.8%), respectively.

CONCLUSIONS

The findings of this study revealed, there is no significant association between the occurrence of virulence genes or source of samples with production of ESBLs among K. pneumoniae isolates.

摘要

背景

肺炎克雷伯菌是引起医院感染的机会致病菌之一，其致病过程涉及多种毒力因子。本研究旨在调查伊朗霍拉马巴德医院临床样本中分离的产超广谱β-内酰胺酶（ESBLs）肺炎克雷伯菌的 entB、mrkD、magA、kfu、iutA、rmpA、ybtS 和 k2 毒力因子基因的流行情况。

方法

在这项描述性、分析性研究中，通过纸片扩散法进行了扩展谱β-内酰胺酶（ESBLs）的表型筛选试验，并用 PCR 法检测肺炎克雷伯菌分离株的毒力因子基因。最后，使用 SPSS 软件（版本 21）对获得的数据进行统计分析，采用卡方检验。

结果

从尿液中分离出 69 株（62.7%）、痰液 16 株（14.5%）、血液 10 株（9.1%）、伤口 9 株（8.2%）、组织 3 株（2.7%）、体液 2 株（1.8%）和导管 1 株（0.9%）的 110 株肺炎克雷伯菌。根据 ESBL 表型筛选试验，57 株（51.8%）肺炎克雷伯菌产 ESBLs。在 57 株 ESBLs 阳性肺炎克雷伯菌中，mrkD、entB、ybtS、iutA、kfu、k2 和 rmpA 基因的发生率分别为 88%（80%）、87%（79.1%）、65%（59.1%）、42%（38.2%）、19%（17.3%）、5%（4.5%）、5%（4.5%）、3%（2.7%）和 2%（1.8%）。