鞣酸作为一种抗菌剂在感染鼠伤寒沙门氏菌的肉鸡中的体内和体外评价。
In vitro and in vivo evaluation of tannic acid as an antibacterial agent in broilers infected with Salmonella Typhimurium.
机构信息
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
出版信息
Poult Sci. 2023 Dec;102(12):102987. doi: 10.1016/j.psj.2023.102987. Epub 2023 Jul 29.
This study was conducted to evaluate tannic acid (TA) as an antibacterial agent against Salmonella Typhimurium in in vitro and in vivo chicken models. The TA formed an inhibitory zone against Salmonella enterica serotypes including S. Typhimurium, S. Enteritidis, and S. Infantis. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of TA against Salmonella Typhimurium nalidixic acid resistant strain (ST) were determined as 40 and 700 μg/mL, respectively. Sublethal doses of TA (5, 10, and 20 μg/mL) restricted swimming and swarming motility and biofilm formation of ST compared to the control group (0 μg/mL) (P < 0.05). The TA-bovine serum albumin (BSA) complex formed at simulated gastric pH (pH 3.75) was hydrolyzed at pH 6.75 and 7.25 (P < 0.05), and the hydrolysis of the TA-BSA complex was stronger at pH 7.25 compared to the pH 6.75 (P < 0.05). The inhibitory zone of the TA-BSA complex against ST at pH 6.75 was lower than TA without BSA at 30 and 60 min (P < 0.05), but not at 120 min (P > 0.1). The inhibitory zone of the TA-BSA complex against ST at pH 7.25 was not decreased at 0, 30, and 60 min compared to TA without BSA (P > 0.1). The recovery rate of TA was 83, 54.8, 10.5, and 19.6% in the gizzard, jejunum, ileum, and ceca, respectively, in broiler chickens. The ST-infected broilers fed 0.25 g/kg of TA had significantly lower unweighted beta diversity distance compared to the sham-challenged control (SCC) and challenged controlled (CC) group on D 21. TA supplementation linearly (P < 0.05) and quadratically (tendency; P = 0.071) reduced relative abundance of the family Peptostreptococcaceae in broilers infected with ST on D 7. TA supplementation linearly (P < 0.05) and quadratically (tendency; P = 0.06) increased the relative abundance of the family Erysipelotrichaceae in broilers infected with ST on D 21. Therefore, TA has potential to be used as an antibacterial agent against the S. Typhimurium infection in broilers.
本研究旨在评估单宁酸（TA）作为一种抗菌剂，用于体外和体内鸡模型中的沙门氏菌 Typhimurium。TA 对包括沙门氏菌肠血清型、肠炎和婴儿血清型在内的沙门氏菌形成了抑菌圈。TA 对耐萘啶酸的沙门氏菌 Typhimurium 菌株（ST）的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为 40 和 700 μg/mL。与对照组（0 μg/mL）相比，亚致死剂量的 TA（5、10 和 20 μg/mL）限制了 ST 的游泳和群集运动以及生物膜形成（P < 0.05）。在模拟胃 pH（pH 3.75）下形成的 TA-牛血清白蛋白（BSA）复合物在 pH 6.75 和 7.25 时被水解（P < 0.05），并且与 pH 6.75 相比，在 pH 7.25 时 TA-BSA 复合物的水解更强（P < 0.05）。在 pH 6.75 时，TA-BSA 复合物对 ST 的抑菌圈低于无 BSA 的 TA 在 30 和 60 分钟时（P < 0.05），但在 120 分钟时则没有（P > 0.1）。在 pH 7.25 时，TA-BSA 复合物对 ST 的抑菌圈在 0、30 和 60 分钟时与无 BSA 的 TA 相比没有降低（P > 0.1）。在肉鸡的肌胃、空肠、回肠和盲肠中，TA 的回收率分别为 83%、54.8%、10.5%和 19.6%。在感染 ST 的肉鸡中，以 0.25 g/kg 剂量添加 TA 的肉鸡在第 21 天的未加权 beta 多样性距离明显低于假攻对照组（SCC）和攻毒对照组（CC）。TA 补充剂呈线性（P < 0.05）和二次方（趋势；P = 0.071）降低了感染 ST 的肉鸡中肠球菌科的相对丰度。在感染 ST 的肉鸡中，TA 补充剂呈线性（P < 0.05）和二次方（趋势；P = 0.06）增加了肠杆菌科的相对丰度。因此，TA 具有作为抗菌剂用于防治肉鸡沙门氏菌 Typhimurium 感染的潜力。
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