College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
Poult Sci. 2013 Jul;92(7):1764-73. doi: 10.3382/ps.2013-03029.
This study investigated the protective effect of β-1,3/1,6-glucan on gut morphology, intestinal epithelial tight junctions, and bacterial translocation of broiler chickens challenged with Salmonella enterica serovar Typhimurium. Ninety Salmonella-free Arbor Acre male broiler chickens were randomly divided into 3 groups: negative control group (NC), Salmonella Typhimurium-infected positive group (PC), and the Salmonella Typhimurium-infected group with dietary 100 mg/kg of β-1,3/1,6-glucan supplementation (T) to determine the effect of β-1,3/1,6-glucan on intestinal barrier function. Salmonella Typhimurium challenge alone significantly decreased villus height (P < 0.001), villus height/crypt depth ratio (P < 0.05), and the number of goblet cells (P < 0.001) in the jejunum at 14 d postinfection (dpi), but significantly increased the number of intestinal secretory IgA (sIgA)-expressing cells at 14 dpi (P < 0.01) and total sIgA levels in the jejunum at 7 (P < 0.05) and 14 dpi (P < 0.01) compared with the unchallenged birds (NC). Dietary β-1,3/1,6-glucan supplementation not only significantly increased villus height, villus height/crypt depth ratio, and the number of goblet cells (P < 0.01), but also increased the number of sIgA-expressing cells (P < 0.05) and sIgA content in the jejunum at 14 dpi (P < 0.01) in birds challenged with Salmonella Typhimurium in comparison with Salmonella Typhimurium challenge alone. β-1,3/1,6-Glucan addition had significant inhibitory effects (P < 0.05) on cecal Salmonella colonization levels and liver Salmonella invasion of the Salmonella Typhimurium-infected birds compared with the PC group. Intestinal tight junction proteins claudin-1, claudin-4, and occludin mRNA expression in the jejunum at 14 dpi was significantly decreased by Salmonella Typhimurium challenge alone (P < 0.01) compared with that of the NC group, whereas β-1,3/1,6-glucan supplementation significantly increased claudin-1 and occludin mRNA expression (P < 0.01) at 14 dpi in the jejunum of the Salmonella Typhimurium-infected birds in comparison with the PC group. Our results indicate that dietary β-1,3/1,6-glucan can alleviate intestinal mucosal barrier impairment in broiler chickens challenged with Salmonella Typhimurium.
本研究旨在探讨 β-1,3/1,6-葡聚糖对感染鼠伤寒沙门氏菌的肉鸡肠道形态、肠道上皮紧密连接和细菌易位的保护作用。将 90 只无沙门氏菌的 Arbor Acre 雄性肉鸡随机分为 3 组：阴性对照组（NC）、鼠伤寒沙门氏菌感染阳性组（PC）和感染组日粮添加 100mg/kgβ-1,3/1,6-葡聚糖（T）组，以确定β-1,3/1,6-葡聚糖对肠道屏障功能的影响。单独感染鼠伤寒沙门氏菌显著降低了感染后 14 天（dpi）空肠的绒毛高度（P < 0.001）、绒毛高度/隐窝深度比（P < 0.05）和杯状细胞数量（P < 0.001），但显著增加了感染后 14 天的肠道分泌型免疫球蛋白 A（sIgA）-表达细胞数量（P < 0.01）和空肠总 sIgA 水平（7dpi：P < 0.05；14dpi：P < 0.01）与未感染组（NC）相比。日粮β-1,3/1,6-葡聚糖的补充不仅显著增加了绒毛高度、绒毛高度/隐窝深度比和杯状细胞数量（P < 0.01），还增加了 sIgA-表达细胞数量（P < 0.05）和感染后 14 天空肠的 sIgA 含量（P < 0.01）与单独感染鼠伤寒沙门氏菌相比。与 PC 组相比，β-1,3/1,6-葡聚糖添加对感染组的盲肠沙门氏菌定植水平和肝脏沙门氏菌侵袭有显著的抑制作用（P < 0.05）。与 NC 组相比，单独感染鼠伤寒沙门氏菌显著降低了感染后 14 天空肠的紧密连接蛋白 Claudin-1、Claudin-4 和 Occludin mRNA 表达（P < 0.01），而β-1,3/1,6-葡聚糖补充显著增加了感染后 14 天空肠中 Claudin-1 和 Occludin mRNA 表达（P < 0.01）与 PC 组相比，感染鼠伤寒沙门氏菌的肉鸡。研究结果表明，日粮β-1,3/1,6-葡聚糖可减轻感染鼠伤寒沙门氏菌的肉鸡肠道黏膜屏障损伤。
