Chaim Sheba Medical Center, The Zabludowicz Research Center for Autoimmune Diseases, Tel Hashomer, Israel.
Ariel University, Ariel, Israel.
Sci Rep. 2023 Oct 16;13(1):17526. doi: 10.1038/s41598-023-44452-5.
Microbial transglutaminase (mTG) is a bacterial survival factor, frequently used as a food additive to glue processed nutrients. As a result, new immunogenic epitopes are generated that might drive autoimmunity. Presently, its contribution to autoimmunity through epitope similarity and cross-reactivity was investigated. Emboss Matcher was used to perform sequence alignment between mTG and various antigens implicated in many autoimmune diseases. Monoclonal and polyclonal antibodies made specifically against mTG were applied to 77 different human tissue antigens using ELISA. Six antigens were detected to share significant homology with mTG immunogenic sequences, representing major targets of common autoimmune conditions. Polyclonal antibody to mTG reacted significantly with 17 out of 77 tissue antigens. This reaction was most pronounced with mitochondrial M2, ANA, and extractable nuclear antigens. The results indicate that sequence similarity and cross-reactivity between mTG and various tissue antigens are possible, supporting the relationship between mTG and the development of autoimmune disorders 150W.
微生物谷氨酰胺转氨酶（mTG）是一种细菌生存因子，常被用作将加工营养物质黏合在一起的食品添加剂。因此，会产生新的免疫原性表位，可能导致自身免疫。目前，通过表位相似性和交叉反应性来研究其对自身免疫的贡献。使用 Emboss Matcher 对 mTG 与多种自身免疫性疾病相关的各种抗原之间进行序列比对。使用 ELISA 检测针对 mTG 特异性的单克隆和多克隆抗体与 77 种不同的人类组织抗原的反应。发现 6 种抗原与 mTG 免疫原性序列具有显著同源性，代表常见自身免疫疾病的主要靶标。针对 mTG 的多克隆抗体与 77 种组织抗原中的 17 种发生明显反应。这种反应在与线粒体 M2、ANA 和可提取核抗原的反应中最为明显。结果表明 mTG 与各种组织抗原之间的序列相似性和交叉反应性是可能的，支持 mTG 与自身免疫性疾病发展之间的关系。
