Anhui University of Science and Technology, 232001, Huainan, Anhui, China.
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 230001, Hefei, Anhui, China.
Cancer Gene Ther. 2023 Dec;30(12):1702-1714. doi: 10.1038/s41417-023-00676-1. Epub 2023 Oct 16.
Glioblastoma is the most common malignant tumor in the central nervous system. The general transcription factor IIE subunit beta (GTF2E2) is crucial for physiological and pathological functions, but its roles in the malignant biological function of glioma remain ambiguous. CCK-8, colony formation assays, TUNEL assays, cell migration assays, wound-healing assays, and xenograft model were established to investigate the biological functions of GTF2E2 both in vitro and in vivo. GTF2E2 was overexpressed in glioma and was associated with poor prognosis of glioma patients. Biological functions of GTF2E2 were investigated both in vitro and in vi0vo by multiple experiments. Moreover, we explored the possible mechanisms of GTF2E2. In our results, we demonstrated that GTF2E2 could be regulated by miR-340-5p directly or indirectly. CCND1 was transcriptionally affected by GTF2E2 and glioma progression was then regulated. Our data presented the overexpression of GTF2E2 in glioma and indicated the association between GTF2E2 and glioma prognosis. GTF2E2 was found to be regulated by miR-340-5p and thus affect downstream gene expressions and glioma progression. Our results indicate that GTF2E2 might be a potential target in the diagnosis and treatments of glioblastoma.
胶质母细胞瘤是中枢神经系统最常见的恶性肿瘤。一般转录因子 IIE 亚基β（GTF2E2）对于生理和病理功能至关重要，但它在胶质瘤恶性生物学功能中的作用仍不清楚。我们建立了 CCK-8、集落形成实验、TUNEL 实验、细胞迁移实验、划痕愈合实验和异种移植模型，以研究 GTF2E2 在体外和体内的生物学功能。GTF2E2 在胶质瘤中过表达，并与胶质瘤患者的预后不良相关。通过多项实验研究了 GTF2E2 的生物学功能。此外，我们还探讨了 GTF2E2 的可能机制。在我们的结果中，我们证明了 GTF2E2 可以直接或间接地被 miR-340-5p 调控。CCND1 受 GTF2E2 的转录影响，从而调节胶质瘤的进展。我们的数据显示了 GTF2E2 在胶质瘤中的过表达，并表明 GTF2E2 与胶质瘤预后之间存在关联。发现 GTF2E2 受 miR-340-5p 调控，从而影响下游基因表达和胶质瘤进展。我们的结果表明，GTF2E2 可能是胶质母细胞瘤诊断和治疗的潜在靶点。
