Department of Oncology, The Third People's Hospital of Linyi, Linyi, Shandong, China.
Department of Personnel, The Third People's Hospital of Linyi, Linyi, Shandong, China.
Bioengineered. 2021 Dec;12(1):9046-9057. doi: 10.1080/21655979.2021.1993715.
The present study was targeted at investigating the effects of long intergenic non-protein coding RNA 1094 on breast cancer (BC) cell proliferation, apoptosis, and cell cycle and its related mechanism. In this study, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were conducted to detect the expressions of LINC01094, microRNA (miRNA, miR)-340-5p, and E2F transcription factor 3 (E2F3) in BC tissues and cells. With transfection, LINC01094 and miR-340-5p expressions were selectively up-regulated or down-regulated in BC cell lines, and then cell proliferation, cell cycle, and apoptosis were examined by cell counting kit-8 (CCK-8), 5-bromo-2'-deoxyuridine (BrdU), and flow cytometry assays. Bioinformatics was utilized to predict the targeted relationships between miR-340-5p and LINC01094, as well as miR-340-5p and E2F3 mRNA 3'-untranslated region (3'UTR), and RNA immunoprecipitation (RIP) assay and dual-luciferase reporter gene assay were employed to validate them. It was revealed that, LINC01094 expression was enhanced in BC cells and tissues, and LINC01094 overexpression promoted BC cell proliferation, accelerated cell cycle progression, and inhibited apoptosis while knocking down LINC01094 worked oppositely. LINC01094 directly targeted miR-340-5p and negatively regulated its expression in BC cells. Besides, E2F3 was substantiated to be the target gene of miR-340-5p, and E2F3 expression could be indirectly and positively modulated by LINC01094. All in all, LINC01094 promotes BC cell proliferation and cell cycle progression and inhibits apoptosis via modulating miR-340-5p/E2F3 molecular axis.
本研究旨在探讨长链非编码 RNA 1094（LINC01094）对乳腺癌（BC）细胞增殖、凋亡和细胞周期的影响及其相关机制。本研究通过 Western blot 和实时定量聚合酶链反应（qRT-PCR）检测 BC 组织和细胞中 LINC01094、微小 RNA（miRNA，miR）-340-5p 和转录因子 E2F3（E2F3）的表达。通过转染，选择性地上调或下调 BC 细胞系中的 LINC01094 和 miR-340-5p 的表达，然后通过细胞计数试剂盒-8（CCK-8）、5-溴-2'-脱氧尿苷（BrdU）和流式细胞术检测细胞增殖、细胞周期和凋亡。生物信息学用于预测 miR-340-5p 与 LINC01094 以及 miR-340-5p 与 E2F3 mRNA 3'-非翻译区（3'UTR）之间的靶向关系，并通过 RNA 免疫沉淀（RIP）实验和双荧光素酶报告基因实验验证它们。结果表明，LINC01094 在 BC 细胞和组织中表达增强，LINC01094 过表达促进 BC 细胞增殖，加速细胞周期进程，抑制凋亡，而敲低 LINC01094 则起到相反的作用。LINC01094 直接靶向 miR-340-5p，并负调控其在 BC 细胞中的表达。此外，E2F3 被证实是 miR-340-5p 的靶基因，E2F3 的表达可以被 LINC01094 间接和正向调节。总之，LINC01094 通过调节 miR-340-5p/E2F3 分子轴促进 BC 细胞增殖、细胞周期进程和抑制凋亡。
