Lin C T, Liu J W, Wu J Y, Dang H, Li C Y, Lam K W
Lab Invest. 1986 Dec;55(6):666-72.
The acid phosphatase (AcP) isoenzyme in a human prostatic cancer cell line was compared to that of prostatic tissue extract by electrophoresis. The major isoenzyme by prostatic tissue extract is the AcP isoenzyme 2, while only AcP isoenzyme 4 (AcP-4) was observed in the human prostatic cancer cell line. A monoclonal antibody specific to AcP-4 was used to investigate the ultrastructural distribution of AcP-4 in a prostatic cancer cell line. The peroxidase staining pattern indicates that AcP-4 is synthesized on bound ribosomes, discharged into the cisternae of rough endoplasmic reticulum, transported to the cisternae of Golgi apparatus for concentration and packaging, and transferred to the secretory vesicles for exocytosis. It is well known that synthesis and secretion of AcP-2 are the major characteristics of the highly differentiated prostatic epithelial cells. The present data demonstrate the loss of this specific function in the prostatic cancer cell line. Instead of AcP-2, the dedifferentiated cancer cell line synthesizes and secretes AcP-4, which is a common AcP isoenzyme of many nonprostatic tissues.
通过电泳将人前列腺癌细胞系中的酸性磷酸酶（AcP）同工酶与前列腺组织提取物中的酸性磷酸酶同工酶进行比较。前列腺组织提取物中的主要同工酶是AcP同工酶2，而在人前列腺癌细胞系中仅观察到AcP同工酶4（AcP - 4）。使用对AcP - 4特异的单克隆抗体研究AcP - 4在前列腺癌细胞系中的超微结构分布。过氧化物酶染色模式表明，AcP - 4在附着核糖体上合成，排入粗面内质网池，转运至高尔基体池进行浓缩和包装，然后转移至分泌小泡进行胞吐作用。众所周知，AcP - 2的合成和分泌是高度分化的前列腺上皮细胞的主要特征。目前的数据表明前列腺癌细胞系中这种特定功能的丧失。去分化的癌细胞系不合成和分泌AcP - 2，而是合成和分泌AcP - 4，AcP - 4是许多非前列腺组织中常见的AcP同工酶。
