Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Antiinflamm Antiallergy Agents Med Chem. 2023;22(2):104-112. doi: 10.2174/0118715230264101230925060355.
The combination of two drugs may lead to better results while reducing the need for each medication.
This study aimed to explore the synergistic benefits of combination therapy by suboptimal dose of niacin (Nic.) and prednisolone (Pred.) in an experimental model of Rheumatoid arthritis (RA).
About 50 male Wistar rats (weighing 150 - 160 grams) were randomly divided into five groups of ten, including healthy and RA groups treated with Nic. (80 mg/kg-orally), or Pred. (2 mg/kg-orally), and/or co-administration of Nic. and Pred. (half doses with each one-orally). RA was induced by the injection of complete Freund's adjuvant into the hind paw of each rat. All treatments were initiated on the fifth day following the induction and continued until day 30 post-induction.
The combined Nic. and Pred. at half doses promoted a significant regression in the severity of the established RA, which is more pronounced than full doses of either drug alone. Combination therapy promoted a reduction in some hematological and biochemical RA parameters, like neutral red uptake by phagocytic cells, myeloperoxidase, nitric oxide, and C-reactive protein, more profound than each drug alone. Combined treatment caused a greater decrease in IFN-γ expression than other treatments in the area of plantar joints. All treatments were effective in increasing the expression of the IL-10 in the area of plantar joints. Prednisolone was less effective in reducing the expression of the TNF-α in the area of plantar joints than the other group.
This combination may be a useful approach to controlling RA.
联合使用两种药物可能会带来更好的效果，同时减少每种药物的需求。
本研究旨在通过亚最优剂量烟酸（Nic.）和泼尼松龙（Pred.）在类风湿关节炎（RA）实验模型中探索联合治疗的协同益处。
大约 50 只雄性 Wistar 大鼠（体重 150-160 克）被随机分为五组，每组 10 只，包括健康组和 RA 组，RA 组分别用 Nic.（80mg/kg-口服）、Pred.（2mg/kg-口服）或 Nic.和 Pred.（半剂量，各一种-口服）联合治疗。RA 通过向每只大鼠的后爪注射完全弗氏佐剂诱导。所有治疗均在诱导后的第 5 天开始，并持续到诱导后的第 30 天。
联合使用半剂量的 Nic.和 Pred.可显著减轻已建立的 RA 的严重程度，其效果比单独使用全剂量的两种药物更明显。联合治疗可降低一些 RA 的血液学和生化参数，如吞噬细胞摄取中性红、髓过氧化物酶、一氧化氮和 C 反应蛋白，其效果比单独使用药物更明显。与其他治疗相比，联合治疗可使 IFN-γ在跖关节区域的表达降低更多。所有治疗均可有效增加跖关节区域的 IL-10 表达。Pred.在降低跖关节区域 TNF-α的表达方面不如其他组有效。
这种联合治疗可能是控制 RA 的一种有效方法。
