Hubei International Science and Technology Cooperation Base of Fish Passage, China Three Gorges University, Yichang, Hubei, China.
College of Hydraulic & Environmental Engineering, China Three Gorges University, Yichang, Hubei, China.
Microsc Res Tech. 2024 Feb;87(2):349-359. doi: 10.1002/jemt.24427. Epub 2023 Oct 16.
The biological synthesis of nanoparticles is an emerging field of study that seeks to synthesize nanoparticles using non-chemical mechanisms such as microorganisms, plants, and animal blood serum. Among these, plants have gained particular attention due to their ease of handling, availability, and ability to synthesize a wide range of nanoparticles. Therefore, the current study aimed to fabricate the silver nanoparticles (AgNPs) using Chinese medicinal plants (CMP) for their possible toxicity in common carp fish (Cyprinus carpio). For this purpose, CMP was dried, ground, and used as a bio-reductive agent. The fabricated AgNPs were characterized and a well dispersed AgNPs were obtained. Moreover, the C. carpio was exposed to the AgNPs for bioaccumulation and histological alterations. The obtained findings revealed that the AgNPs were mostly accumulated in the intestines followed by the gills, muscles, liver, and brain. The accumulated AgNPs caused histological alterations in gills and intestines at the highest concentration (0.08 mg/L). However, very less alterations were caused by the lowest concentration, especially in the intestine. In conclusion, further in-depth research is needed to determine the risks associated with the usage of nanoparticles to reveal their harmful impacts on fish and the aquatic environment. HIGHLIGHTS: The biological fabrication of AgNPs is considered eco-friendly. Chinese medicinal plants play a significant role in AgNPs synthesis. AgNPs have excellent antibacterial activity. AgNPs are bioaccumulated in various organs of fish.
纳米粒子的生物合成是一个新兴的研究领域，旨在利用非化学机制合成纳米粒子，如微生物、植物和动物血清。在这些方法中，由于植物易于操作、易于获取以及能够合成广泛范围的纳米粒子，因此受到了特别关注。因此，本研究旨在使用中草药（CMP）来制备银纳米粒子（AgNPs），并研究其对鲤鱼（Cyprinus carpio）的可能毒性。为此，将 CMP 干燥、研磨并用作生物还原剂。对制备的 AgNPs 进行了表征，得到了分散良好的 AgNPs。此外，还将 C. carpio 暴露于 AgNPs 中以进行生物累积和组织学改变的研究。研究结果表明，AgNPs 主要在肠道中积累，然后在鳃、肌肉、肝脏和大脑中积累。在最高浓度（0.08mg/L）下，AgNPs 在鳃和肠道中引起了组织学改变。然而，在最低浓度下，AgNPs 引起的改变非常少，尤其是在肠道中。总之，需要进一步深入研究以确定与使用纳米粒子相关的风险，以揭示它们对鱼类和水生环境的有害影响。重点：AgNPs 的生物制备被认为是环保的。中草药在 AgNPs 合成中起着重要作用。AgNPs 具有出色的抗菌活性。AgNPs 在鱼类的各种器官中累积。
