Kakakhel Mian Adnan, Zaheer Ud Din Syed, Wang Wentao
MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
International School for Optoelectronic Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Microsc Res Tech. 2022 Apr;85(4):1282-1288. doi: 10.1002/jemt.23994. Epub 2021 Nov 20.
At present, nanotechnology and nanomaterials are being emerged very quickly and gained the attention of researchers due to their frequent application history, especially their antibacterial effects against certain bacterial isolates. Therefore, the present study was aimed to check the antibacterial influence and toxic effects of the market available (Chemical Fabricated) silver nanoparticles (AgNPs). For this purpose, the AgNPs were obtained from the local market and used against fish pathogenic bacterial species. The highest zone of inhibition was observed against Aeromonas hydrophila (27.53 ± 0.69). Moreover, the AgNPs were exposed to the common carp fish for toxicity and toxic effects. The highest mortality was seen at the highest concentration (0.09 mg/L) of AgNPs. Finally, the AgNPs mainly were accumulated in the liver, followed by the intestine, gills, and muscles. The intestine absorbed the nanomaterials; therefore, it is critical to check the influence of these AgNPs on the fish intestinal bacterial community via MiSeq Illumina Sequencing.
目前，纳米技术和纳米材料发展迅速，因其广泛的应用历史，尤其是对某些细菌分离株的抗菌作用而受到研究人员的关注。因此，本研究旨在检测市售（化学合成）银纳米颗粒（AgNPs）的抗菌影响和毒性作用。为此，从当地市场获取AgNPs，并将其用于对抗鱼类致病细菌物种。观察到对嗜水气单胞菌的抑菌圈最大（27.53±0.69）。此外，将AgNPs暴露于鲤鱼以检测其毒性和毒效。在AgNPs最高浓度（0.09 mg/L）时观察到最高死亡率。最后，AgNPs主要积聚在肝脏，其次是肠道、鳃和肌肉。肠道吸收了纳米材料；因此，通过MiSeq Illumina测序检查这些AgNPs对鱼类肠道细菌群落的影响至关重要。
