齐墩果酸脂质体对顺铂诱导的小鼠少弱精子症的保护作用

[Protective effect of oleanolic acid liposomes on cisplatin-induced oligoasthenospermia in mice].

作者信息

Zhao Jun-Xiu, Chen Zhen-Yu, Tan Zhen-Yu, Li Ai-Jin, Xi Yan-Li

机构信息

School of Public Health, Jilin Medical College, Jilin, Jilin 132013, China.

School of Clinical Medicine, Jilin Medical College, Jilin, Jilin 132013, China.

出版信息

Zhonghua Nan Ke Xue. 2022 Nov;28(11):970-975.

PMID:37846112

Abstract

OBJECTIVE

To study the protective effect of oleanolic acid liposomes (OA-Lips) on cisplatin-induced oligoasthenospermia (COAS) in mice.

METHODS

Sixty ICR mice were randomly divided into a normal control, a COAS model control, a positive control and a low-, a medium- and a high-dose OA-Lips group. The animals in the low-, medium- and high-dose OA-Lips and positive control groups were given intragastrically OA-Lips solution at 25, 50, and 100 mg/kg/d and vitamin E at 50 mg/kg/d, respectively. On the 28th day, the mice in the COAS model control, positive control and OA-Lips groups were injected intraperitoneally with cisplatin solution at 10 mg/kg, while those in the normal control group with the same dose of normal saline. Three days after administration, all the mice were sacrificed and their testis tissues collected for detection of the semen parameters and observation of the testicular morphology.

RESULTS

Both the percentage of motile sperm and sperm concentration were significantly increased in the high-dose OA-Lips group (P < 0.05). HE staining showed that OA-Lips remarkably improved the damaged testis tissue (P < 0.05) and protected the seminiferous tubules and interstitial cells. The percentage of progressively motile sperm (PMS) and the curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat-cross frequency (BCF) of sperm were gradually increased in a dose-dependent manner in the OA-Lips groups. The serum T level was significantly higher (P < 0.05) in the OA-Lips-treated mice than in the COAS model controls while the percentage of morphologically abnormal sperm (MAS) markedly lower in the high-dose OA-Lips group than in the model control, positive control and low-dose OA-Lips groups (P < 0.05).

CONCLUSION

OA-Lips can relieve oligoaspermia and improve the productive ability of mice.

摘要

目的

研究齐墩果酸脂质体（OA-Lips）对顺铂诱导的小鼠少弱精子症（COAS）的保护作用。

方法

将60只ICR小鼠随机分为正常对照组、COAS模型对照组、阳性对照组以及低、中、高剂量OA-Lips组。低、中、高剂量OA-Lips组和阳性对照组动物分别按25、50和100 mg/kg/d灌胃给予OA-Lips溶液，阳性对照组按50 mg/kg/d灌胃给予维生素E。第28天，COAS模型对照组、阳性对照组和OA-Lips组小鼠腹腔注射10 mg/kg顺铂溶液，正常对照组小鼠注射相同剂量的生理盐水。给药3天后，处死所有小鼠，采集睾丸组织检测精液参数并观察睾丸形态。

结果

高剂量OA-Lips组精子活动率和精子浓度均显著升高（P < 0.05）。苏木精-伊红（HE）染色显示，OA-Lips显著改善受损的睾丸组织（P < 0.05），保护生精小管和间质细胞。OA-Lips组中，精子的前向运动精子百分比（PMS）、曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）、线性度（LIN）、直线性（STR）、摆动性（WOB）、头部侧摆幅度（ALH）和鞭打频率（BCF）呈剂量依赖性逐渐升高。OA-Lips处理的小鼠血清睾酮（T）水平显著高于COAS模型对照组（P < 0.05），高剂量OA-Lips组形态异常精子百分比（MAS）显著低于模型对照组、阳性对照组和低剂量OA-Lips组（P < 0.05）。