Huang Qianqian, Wu Haiying, Qin Xiaoming
Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
National Research and Development Branch Center for Shellfish Processing, Zhanjiang 524088, China.
Foods. 2023 Aug 28;12(17):3236. doi: 10.3390/foods12173236.
extract (PGE) has a variety of biological activities. However, its ameliorative effect on and exact working mechanism in male sexual dysfunction are still poorly understood. This study aims to evaluate the ameliorative effect of PGE on paroxetine (PRX)-induced sexual dysfunction in male mice and uses molecular docking technology to investigate its underlying mechanism. In this work, PRX-induced sexual dysfunction was caused and PGE was gavaged in mice for 28 days. The results show that PGE significantly improved the sexual performance of mice and reduced the damage to testicular tissues. Further studies showed that PGE restored serum sex hormones to normal levels and increased nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) levels as well as nitric oxide synthase (NOS) activity in penile tissues, while also decreasing phosphodiesterase-5 (PDE-5) activity, thereby maintaining normal penile erection in mice. In addition, PGE improved the activities of enzymes (LDH, ACP, and ALP) related to energy metabolism in the testis and significantly increased sperm count and viability in mice. Furthermore, the molecular docking results show that all eight compounds in PGE could form a stable complex with PDE-5 and inhibit the activity of PDE-5. In conclusion, PGE had an ameliorative effect on PRX-induced sexual dysfunction, suggesting that PGE has a potential protective effect on male sexual health.
前列腺素E（PGE）具有多种生物学活性。然而，其对男性性功能障碍的改善作用及确切作用机制仍知之甚少。本研究旨在评估PGE对帕罗西汀（PRX）诱导的雄性小鼠性功能障碍的改善作用，并利用分子对接技术探究其潜在机制。在本研究中，诱导小鼠出现PRX诱导的性功能障碍，并对其灌胃PGE 28天。结果表明，PGE显著改善了小鼠的性行为表现，并减轻了对睾丸组织的损伤。进一步研究表明，PGE使血清性激素恢复至正常水平，并提高了阴茎组织中一氧化氮（NO）和环磷酸鸟苷（cGMP）水平以及一氧化氮合酶（NOS）活性，同时还降低了磷酸二酯酶-5（PDE-5）活性，从而维持小鼠阴茎的正常勃起。此外，PGE提高了睾丸中与能量代谢相关的酶（乳酸脱氢酶、酸性磷酸酶和碱性磷酸酶）的活性，并显著增加了小鼠的精子数量和活力。此外，分子对接结果表明，PGE中的所有八种化合物均可与PDE-5形成稳定复合物并抑制PDE-5的活性。综上所述，PGE对PRX诱导的性功能障碍具有改善作用，提示PGE对男性性健康具有潜在保护作用。
