College of Plant Protection, Nanjing Agricultural University, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, China.
Bayer Cropscience (China) Co., Ltd., Hangzhou, China.
Insect Sci. 2024 Jun;31(3):835-846. doi: 10.1111/1744-7917.13282. Epub 2023 Oct 17.
The rice stem borer (RSB), Chilo suppressalis, a notorious rice pest in China, has evolved a high resistance level to commonly used insecticides. Tetraniliprole, a new anthranilic diamide insecticide, effectively controls multiple pests, including RSB. However, the potential resistance risk of RSB to tetraniliprole is still unknown. In this study, the tetraniliprole-selection (Tet-R) strain was obtained through 10 continuous generations of selection with tetraniliprole 30% lethal concentration (LC). The realized heritability (h) of the Tet-R strain was 0.387, indicating that resistance of RSB to tetraniliprole developed rapidly under the continuous selection of tetraniliprole. The Tet-R strain had a high fitness cost (relative fitness = 0.53). We established the susceptibility baseline of RSB to tetraniliprole (lethal concentration at LC = 0.727 mg/L) and investigated the resistance level of 6 field populations to tetraniliprole. All tested strains that had resistance to chlorantraniliprole exhibited moderate- to high-level resistance to tetraniliprole (resistance ratio = 27.7-806.8). Detection of ryanodine receptor (RyR) mutations showed that the Y4667C, Y4667D, I4758M, and Y4891F mutations were present in tested RSB field populations. RyR mutations were responsible for the cross-resistance between tetraniliprole and chlorantraniliprole. Further, the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9-mediated genome-modified flies were used to study the contribution of RyR mutations to tetraniliprole resistance. The order of contribution of a single RyR mutation to tetraniliprole resistance was Y4667D > G4915E > Y4667C ≈ I4758M > Y4891F. In addition, the I4758M and Y4667C double mutations conferred higher tetraniliprole resistance than single Y4667C mutations. These results can guide resistance management practices for diamides in RSB and other arthropods.
水稻螟虫（RSB），二化螟，是中国一种臭名昭著的水稻害虫，已经对常用杀虫剂产生了高度的抗药性。四氯虫酰胺，一种新型邻苯二甲酰胺类杀虫剂，可有效防治包括 RSB 在内的多种害虫。然而，RSB 对四氯虫酰胺产生潜在抗药性的风险仍未知。在本研究中，通过用四氯虫酰胺 30%致死浓度（LC）连续选择 10 代，获得了对四氯虫酰胺具有抗性的（Tet-R）品系。Tet-R 品系的现实遗传力（h）为 0.387，表明 RSB 对四氯虫酰胺的抗药性在四氯虫酰胺的连续选择下迅速发展。Tet-R 品系的适应度代价较高（相对适合度=0.53）。我们建立了 RSB 对四氯虫酰胺的敏感性基线（LC 致死浓度=0.727mg/L），并调查了 6 个田间种群对四氯虫酰胺的抗性水平。所有对氯虫酰胺表现出中至高抗药性的测试菌株对四氯虫酰胺也表现出了中等至高抗药性（抗性比=27.7-806.8）。检测到肌质网钙释放通道（RyR）突变显示，在所测试的 RSB 田间种群中存在 Y4667C、Y4667D、I4758M 和 Y4891F 突变。RyR 突变是四氯虫酰胺和氯虫酰胺产生交叉抗性的原因。此外，使用成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关蛋白 9 介导的基因组修饰果蝇来研究 RyR 突变对四氯虫酰胺抗性的贡献。单个 RyR 突变对四氯虫酰胺抗性的贡献顺序为 Y4667D> G4915E> Y4667C≈I4758M> Y4891F。此外，I4758M 和 Y4667C 双突变比单个 Y4667C 突变赋予更高的四氯虫酰胺抗性。这些结果可以指导二酰胺类杀虫剂在防治 RSB 和其他节肢动物中的抗性管理实践。
