Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
J Econ Entomol. 2019 May 22;112(3):1348-1353. doi: 10.1093/jee/toz001.
Chlorantraniliprole, an anthranilic diamide insecticide, is widely used for controlling lepidopteran pests, because of its high insecticidal activity. However, overuse of chlorantraniliprole has led to the selection of resistance in many insect pests, including Chilo suppressalis (Lepidoptera:Crambidae), one of the most damaging rice pests in China. In this study, resistance levels to chlorantraniliprole for C. suppressalis was surveyed from eight populations of three provinces in China. The levels of resistance were ranged from 34.4-fold to 284.0-fold compared with a susceptible population. Then, a 15402 bp fragment of the full-length cDNA of ryanodine receptor gene (CsRyR) from the XS strain, the highest resistant population, and a 1992 bp fragment of CsRyR cDNA encoding the carboxyl-terminal of CsRyR gene from the other seven populations were sequenced. A common previously identified mutation that was associated with chlorantraniliprole resistance against C. suppressalis, G4910E, was not detected in any of the eight populations in this study. However, another mutation I4758M was found in all seven resistant populations. Furthermore, the relative mRNA expression levels of CsRyR gene in the seven resistant populations were all reduced compared with susceptible strain. Our study provides new insights into the basis of monitoring the development of resistance and the mechanism of resistance to chlorantraniliprole in C. suppressalis.
氯虫苯甲酰胺，一种邻苯二甲酰胺类杀虫剂，由于其高效的杀虫活性而被广泛用于防治鳞翅目害虫。然而，氯虫苯甲酰胺的过度使用导致许多害虫对其产生了抗性，包括斜纹夜蛾（鳞翅目：夜蛾科），这是中国最具破坏性的水稻害虫之一。在这项研究中，我们对来自中国三个省份的八个斜纹夜蛾种群进行了氯虫苯甲酰胺抗性水平的调查。与敏感种群相比，抗性水平的范围从 34.4 倍到 284.0 倍不等。然后，我们对来自最高抗性种群 XS 菌株的全长 ryanodine 受体基因（CsRyR）的 15402 bp 片段和来自其他七个种群的编码 CsRyR 基因羧基末端的 1992 bp 片段进行了测序。我们没有在这八个种群中检测到之前与斜纹夜蛾对氯虫苯甲酰胺抗性相关的常见突变 G4910E。然而，我们发现了另一个突变 I4758M，它存在于所有七个抗性种群中。此外，与敏感种群相比，CsRyR 基因在这七个抗性种群中的相对 mRNA 表达水平都降低了。我们的研究为监测斜纹夜蛾对氯虫苯甲酰胺抗性的发展和抗性机制提供了新的见解。
