Ullah Farman, G Guru-Pirasanna-Pandi, Gul Hina, Panda Rudra Madhab, Murtaza Ghulam, Zhang Zhijun, Huang Jun, Li Xiaowei, Desneux Nicolas, Lu Yaobin
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Protection Division, ICAR-National Rice Research Institute, Cuttack, Odisha, India.
Front Plant Sci. 2025 Apr 28;16:1573634. doi: 10.3389/fpls.2025.1573634. eCollection 2025.
Cyantraniliprole, a second-generation anthranilic diamide, is widely used to manage lepidopteran pests, including the invasive tomato pinworm (Meyrick). However, cyantraniliprole's resistance mechanisms and associated fitness costs in remain underexplored. Here, we investigated the fitness costs and resistance mechanisms of cyantraniliprole-resistant strain (CyanRS) via nanocarrier-mediated RNA interference (RNAi). Results showed that the egg incubation period and developmental durations of larval and pupal stages were significantly prolonged in the CyanRS population of compared to the susceptible strain (SS). Further, the adult emergence, longevities of male and female, fecundity, and hatching rate were significantly reduced in CyanRS individuals. The mRNA expression levels of cytochrome b5 reductase () and cytochrome P450 () were analyzed using RT-qPCR to explore their potential involvement in cyantraniliprole resistance in . Phylogenetic and motif analysis of and indicated their evolutionary and functional conservation with other insect species, especially Lepidopterans. Notably, nanocarrier-encapsulated dsRNA targeting and genes significantly reduced their expression levels. Further, the activity of cytochrome P450 was substantially decreased after the knockdown of the and genes. This increased susceptibility of the resistant population of to cyantraniliprole, leading to a higher mortality rate than the controls. These findings show that and might play a key role in cyantraniliprole resistance evolution in . The current study provides in-depth insights to understand the underlying mechanisms of cyantraniliprole resistance in this key invasive herbivore.
氰虫酰胺是一种第二代邻氨基苯甲酰胺类杀虫剂，广泛用于防治鳞翅目害虫，包括入侵性番茄潜叶蛾（麦氏潜叶蛾）。然而，氰虫酰胺的抗性机制及其相关的适合度代价仍未得到充分研究。在此，我们通过纳米载体介导的RNA干扰（RNAi）研究了氰虫酰胺抗性品系（CyanRS）的适合度代价和抗性机制。结果表明，与敏感品系（SS）相比，CyanRS群体的卵孵化期以及幼虫和蛹期的发育持续时间显著延长。此外，CyanRS个体的成虫羽化率、雌雄成虫寿命、繁殖力和孵化率均显著降低。使用RT-qPCR分析了细胞色素b5还原酶（）和细胞色素P450（）的mRNA表达水平，以探讨它们在氰虫酰胺抗性中的潜在作用。对和的系统发育和基序分析表明，它们与其他昆虫物种，尤其是鳞翅目昆虫具有进化和功能保守性。值得注意的是，靶向和基因的纳米载体包裹的dsRNA显著降低了它们的表达水平。此外，和基因敲低后，细胞色素P450的活性大幅下降。这增加了抗性群体对氰虫酰胺的敏感性，导致死亡率高于对照组。这些发现表明，和可能在氰虫酰胺抗性进化中起关键作用。本研究为理解这种关键入侵性食草害虫对氰虫酰胺抗性的潜在机制提供了深入见解。
