Cosan Semih, İnam Onur, Kaya Bengisu, Atilla Pergin, Ocal Osman, Arat Anil, Karakaya Jale, Arat Yonca O
Department of Ophthalmology, Faculty of Medicine, Hacettepe University, 06230, Sıhhiye, Ankara, Turkey.
Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Int Ophthalmol. 2023 Dec;43(12):4985-4990. doi: 10.1007/s10792-023-02901-0. Epub 2023 Oct 17.
Periorbital fat atrophy is a known side effect of topical prostaglandin analogs (PA). This side effect may have implications in the treatment of diseases like thyroid orbitopathy. In this in vivo study we aimed to evaluate the effects of retrobulbar injection of three different PAs on orbital fat.
Eighteen adult male Wistar-albino rats were divided into three groups of six animals. 0.1 ml of 0.03% bimatoprost, 0.005% latanoprost, or 0.005% travoprost was injected into the right orbits and saline was injected into the left orbits, as controls. Both orbits were exenterated after 3 weeks. Histological cross-sections were analyzed using ImageJ image analysis software. Intraconal adipocyte density was calculated.
There was no significant difference in the adipocyte density between the PA injected orbits and the control side in each of the three groups. When calculations from all three groups were analyzed together, again the difference in the adipocyte density between the PA injected orbits and the control side was not significant.
No significant fat atrophy was noted in this rat model three weeks after retrobulbar injection of PAs. To evaluate retrobulbar injection of PA as a potential therapy for orbital diseases with fat proliferation, in vivo studies in different animal models, higher concentrations of PA, or longer follow-up duration are required.
眶周脂肪萎缩是局部应用前列腺素类似物（PA）已知的副作用。这种副作用可能对甲状腺眼病等疾病的治疗有影响。在这项体内研究中，我们旨在评估球后注射三种不同PA对眼眶脂肪的影响。
将18只成年雄性Wistar白化大鼠分为三组，每组6只动物。将0.1 ml 0.03%的比马前列素、0.005%的拉坦前列素或0.005%的曲伏前列素注入右眼眶，作为对照，左眼眶注入生理盐水。3周后将双侧眼眶内容物剜除。使用ImageJ图像分析软件对组织学横截面进行分析。计算眶内脂肪细胞密度。
在三组中的每一组中，注射PA的眼眶与对照侧的脂肪细胞密度均无显著差异。当对所有三组的计算结果一起分析时，注射PA的眼眶与对照侧的脂肪细胞密度差异仍然不显著。
在该大鼠模型中，球后注射PA三周后未观察到明显的脂肪萎缩。要评估球后注射PA作为脂肪增生性眼眶疾病的潜在治疗方法，需要在不同动物模型中进行体内研究、使用更高浓度的PA或延长随访时间。
