Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong, P. R. China.
Electrophoresis. 2023 Nov;44(21-22):1714-1724. doi: 10.1002/elps.202300059. Epub 2023 Oct 17.
Correctly inferring the tissue origin types of forensic-relevant body fluids left at a crime scene is beneficial for reconstructing a crime scene. However, it is still a challenge to accurately identify different kinds of body fluids at a crime scene. Shorter sequence length and anti-degradation microRNA (miRNA) can be used to infer the tissue sources of biological fluid traces, but a limited number of miRNAs are tissue specific. The application of messenger RNA (mRNA) has been confirmed by different studies based on its high tissue specificity. According to the differential expression features of mRNA or miRNA in forensically relevant body fluids, this study developed a simultaneously reversed mRNA and miRNA system and then used these two types of RNAs for the determinations of five common kinds of body fluids. Compared with previously reported single kind of mRNA or miRNA assay, the combined mRNA and miRNA system showed good advantages for human body fluid identifications, especially it could be applied in mixed samples. In conclusion, the obtained results indicated that this combined mRNA and miRNA system might provide a scientific and accurate reference for body fluid identifications.
正确推断法医相关体液在犯罪现场的组织来源类型有助于重建犯罪现场。然而，准确识别犯罪现场的不同种类的体液仍然是一个挑战。较短的序列长度和抗降解的 microRNA（miRNA）可用于推断生物流体痕迹的组织来源，但有限数量的 miRNA 是组织特异性的。信使 RNA（mRNA）的应用已被不同的研究基于其高组织特异性所证实。根据法医相关体液中 mRNA 或 miRNA 的差异表达特征，本研究开发了一个同时反转的 mRNA 和 miRNA 系统，然后使用这两种类型的 RNA 来鉴定五种常见的体液。与之前报道的单一类型的 mRNA 或 miRNA 分析相比，组合的 mRNA 和 miRNA 系统在人体体液鉴定方面表现出良好的优势，尤其是在混合样本中也能适用。总之，研究结果表明，该组合的 mRNA 和 miRNA 系统可能为体液鉴定提供科学准确的参考。
