College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 7):127417. doi: 10.1016/j.ijbiomac.2023.127417. Epub 2023 Oct 16.
Chicken embryo development is a dynamic process. However, no detailed information is available about the protein abundance changes associated with the lipid mechanism and antioxidant enzyme activity during the egg embryo development. Thus, in the present study, an TMT-based proteomic approach was used to quantify protein abundance changes at different stages of chicken embryonic development. A total of 289 significantly differentially abundant hepatic proteins were quantified, of which 180 were upregulated and 109 were downregulated in the comparison of Day 20 with Day 12 in chicken embryos. Pathway analysis showed that metabolic pathways were the most highly enriched pathways, followed by arachidonic acid metabolism and steroid biosynthesis. Integration of proteomic-based studies profiling of three incubation stages revealed that the two compare groups (Day 12 vs Day 20 and Day 16 vs Day 20) shared some key differentially abundant proteins (DAPs), including LBFABP, FABP5, CYP4V2, PDCD4, LAL, APOA1, APOA4, SAA, FABP2, ACBSG2, FABP2, CYP51A1, and FBXO9. The STRING database and GO analysis results showed that there was close connectivity between APOA4, LBFABP, SERPINC1, APOA1, FGB, FGA, ANGPTL3 and these proteins were involved in the oxidation-reduction process, lipid transport, iron ion, heme, and lipid binding. Importantly, APOA4, FABP2, and CYP51A1 might be key factors to control fat deposition and antioxidant enzyme activity during chicken embryonic development. These findings will facilitate a better understanding of antioxidant and lipid mechanisms in chicken embryo and these DAPs can be further investigated as candidate markers to predict lipid deposition and the activity of antioxidant enzymes.
鸡胚胎发育是一个动态的过程。然而，关于与脂质机制相关的蛋白质丰度变化以及抗氧化酶活性在鸡蛋胚胎发育过程中的详细信息尚不清楚。因此，本研究采用基于 TMT 的蛋白质组学方法来定量分析鸡胚胎发育不同阶段的蛋白质丰度变化。共定量了 289 种差异表达的肝蛋白，其中 180 种在鸡胚胎第 20 天与第 12 天的比较中上调，109 种下调。通路分析表明，代谢途径是最富集的途径，其次是花生四烯酸代谢和甾体生物合成。整合三个孵化阶段的蛋白质组学研究结果表明，两个比较组（第 12 天与第 20 天和第 16 天与第 20 天）共享一些关键的差异表达蛋白（DAP），包括 LBFABP、FABP5、CYP4V2、PDCD4、LAL、APOA1、APOA4、SAA、FABP2、ACBSG2、FABP2、CYP51A1 和 FBXO9。STRING 数据库和 GO 分析结果表明，APOA4、LBFABP、SERPINC1、APOA1、FGB、FGA、ANGPTL3 之间存在紧密的连接，这些蛋白参与氧化还原过程、脂质转运、铁离子、血红素和脂质结合。重要的是，APOA4、FABP2 和 CYP51A1 可能是控制鸡胚胎发育过程中脂肪沉积和抗氧化酶活性的关键因素。这些发现将有助于更好地理解鸡胚胎中的抗氧化和脂质机制，这些 DAP 可以进一步作为候选标志物来预测脂质沉积和抗氧化酶的活性。
