Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, 300350, China.
Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, Weifang, Shan Dong, 261000, China.
BMC Cancer. 2023 Oct 17;23(1):989. doi: 10.1186/s12885-023-11502-0.
Long noncoding RNAs (lncRNAs) are considered key players in the formation and development of tumors. Herein, Gene Expression Profiling Interactive Analysis (GEPIA) was employed as a bioinformatics technology. LINC02587 is differentially expressed in bladder urothelial cancer, glioblastoma, lung adenocarcinoma, lung SCC, melanoma, and other tumor tissue and cells. However, its impact on the emergence of glioma and its mechanism is remaining elusive.
Some of the in vitro assays employed in this study were the CCK-8 / Annexin-V / Transwell assays, colony formation, and wound healing, together with Western blot (WB) evaluation. MSP / BSP assays were employed for assessing the CpG island's methylation status in the LINC02587 promoter. Through transcriptome, ferroptosis-related experiments, and WB evaluation, it was confirmed that LINC02587 is correlated with the regulation of ferroptosis in tumor cells, and CoQ-Fsp1 is one of its regulatory pathways. Moreover, the underlined in-vitro results were further validated by in-vivo studies.
The current study shows that the promoter sequence of LINC02587 is regulated by methylation. The silencing of LINC02587 can inhibit cellular proliferative, migrative, and invasive properties, and induce ferroptosis within gliomas through the CoQ-FSP1 pathway.
LINC02587 is likely to be a novel drug target in treating glioma.
长链非编码 RNA（lncRNA）被认为是肿瘤形成和发展的关键因素。本研究采用基因表达谱分析交互分析（GEPIA）作为生物信息学技术。LINC02587 在膀胱癌、胶质母细胞瘤、肺腺癌、肺鳞癌、黑色素瘤等肿瘤组织和细胞中差异表达。然而，其对胶质瘤发生的影响及其机制尚不清楚。
本研究采用 CCK-8/Annexin-V/Transwell 检测、集落形成、划痕愈合实验和 Western blot（WB）评估等体外实验。采用 MSP/BSP 检测试剂盒评估 LINC02587 启动子区 CpG 岛的甲基化状态。通过转录组学、铁死亡相关实验和 WB 评估，证实 LINC02587 与肿瘤细胞铁死亡的调节有关，CoQ-Fsp1 是其调节途径之一。此外，还通过体内研究进一步验证了基础的体外结果。
本研究表明，LINC02587 的启动子序列受甲基化调控。沉默 LINC02587 可以通过 CoQ-FSP1 途径抑制胶质瘤细胞的增殖、迁移和侵袭能力，并诱导铁死亡。
LINC02587 可能成为治疗胶质瘤的新靶点。
