Jiao Yuling, Wang Ying
State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
Peking-Tsinghua Center for Life Sciences, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Biodes Res. 2023 Oct 16;5:0020. doi: 10.34133/bdr.0020. eCollection 2023.
Rapid advances in DNA synthesis techniques have allowed the assembly and engineering of viral and microbial genomes. Multicellular eukaryotic organisms, with their larger genomes, abundant transposons, and prevalent epigenetic regulation, present a new frontier to synthetic genomics. Plant synthetic genomics have long been proposed, and exciting progress has been made using the top-down approach. In this perspective, we propose applying bottom-up genome synthesis in multicellular plants, starting from the model moss , in which homologous recombination, DNA delivery, and regeneration are possible, although further optimizations are necessary. We then discuss technical barriers, including genome assembly and plant transformation, associated with synthetic genomics in seed plants.
DNA合成技术的飞速发展使得病毒和微生物基因组的组装与工程改造成为可能。多细胞真核生物具有更大的基因组、丰富的转座子以及普遍存在的表观遗传调控，为合成基因组学带来了新的前沿领域。植物合成基因组学的设想由来已久，并且通过自上而下的方法已经取得了令人振奋的进展。从这个角度来看，我们建议从模式苔藓开始，在多细胞植物中应用自下而上的基因组合成方法，在苔藓中虽然还需要进一步优化，但同源重组、DNA导入和再生都是可行的。然后我们讨论了与种子植物合成基因组学相关的技术障碍，包括基因组组装和植物转化。
