Rogala-Wielgus Dorota, Majkowska-Marzec Beata, Zieliński Andrzej, Roszek Katarzyna, Liszewska Malwina
Division of Biomaterials Technology, Institute of Manufacturing and Materials Technology, Faculty of Mechanical Engineering and Ship Technology, Gdansk University of Technology 11 Narutowicza Str. 80-233 Gdańsk Poland
Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń Lwowska 1 Str. 87-100 Toruń Poland.
RSC Adv. 2023 Oct 16;13(43):30108-30117. doi: 10.1039/d3ra05331h. eCollection 2023 Oct 11.
Multi-wall carbon nanotube (MWCNT) coatings are gaining increasing interest because of their special properties used in many science fields. The titania coatings are known for their improvement of osteoblast adhesion, thus changing the surface architecture. Bi-layer coatings comprising 0.25 wt% of the MWCNTs and 0.30 wt% of titania (anatase structure) were synthesized in a two-stage procedure using the electrophoretic deposition method (EPD). The MWCNT and TiO coatings were deposited with voltage and time parameters, respectively, of 20 V and 0.5 min, and 50 V and 4 min. EDS, AFM, SEM, Raman spectroscopy, nano-scratch test, potentiodynamic corrosion tests, wettability studies, and cytotoxicity determined with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test on human dermal fibroblasts (HDF) and mouse osteoblast precursors (MC3T3), and lactate dehydrogenase (LDH) activity test were carried out on examined surfaces. The prepared MWCNT/TiO coating is uniformly distributed by MWCNTs and agglomerated by TiO particles of size ranging from 0.1 to 3 μm. Raman spectroscopy confirmed the anatase structure of the TiO addition and showed typical peaks of the MWCNTs. The MWCNT/TiO coating had higher roughness, higher adhesion strength, and improved corrosion resistance compared to the MWCNT basic coating. The results of biological tests proved that physicochemical properties of the surface, such as high porosity and wettability of MWCNT/TiO-coated material, would support cell adhesion, but toxic species could be released to the culture medium, thus resulting in a decrease in proliferation.
多壁碳纳米管（MWCNT）涂层因其在许多科学领域中使用的特殊性能而越来越受到关注。二氧化钛涂层以其对成骨细胞粘附的改善而闻名，从而改变了表面结构。采用电泳沉积法（EPD），通过两步法合成了由0.25 wt%的MWCNT和0.30 wt%的二氧化钛（锐钛矿结构）组成的双层涂层。MWCNT涂层和TiO涂层分别在20 V和0.5 min以及50 V和4 min的电压和时间参数下沉积。对所检测的表面进行了能谱分析（EDS）、原子力显微镜（AFM）、扫描电子显微镜（SEM）、拉曼光谱、纳米划痕试验、动电位腐蚀试验、润湿性研究，并用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）试验测定了对人皮肤成纤维细胞（HDF）和小鼠成骨细胞前体（MC3T3）的细胞毒性，以及乳酸脱氢酶（LDH）活性试验。制备的MWCNT/TiO涂层由MWCNT均匀分布，并由尺寸范围为0.1至3μm的TiO颗粒团聚而成。拉曼光谱证实了TiO添加剂的锐钛矿结构，并显示了MWCNT的典型峰。与MWCNT基础涂层相比，MWCNT/TiO涂层具有更高的粗糙度、更高的粘附强度和更好的耐腐蚀性。生物学测试结果证明，MWCNT/TiO涂层材料的高孔隙率和润湿性等表面物理化学性质有利于细胞粘附，但可能会有有毒物质释放到培养基中，从而导致细胞增殖减少。
