Department of Chemical, Biological, and Materials Engineering, University of South Florida, Tampa, FL, United States of America.
Department of Electrical Engineering, University of South Florida, Tampa, FL, United States of America.
PLoS One. 2023 Oct 18;18(10):e0293035. doi: 10.1371/journal.pone.0293035. eCollection 2023.
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses to the cells of interest. Corona charge was investigated in this study as an alternative to traditional methods as a means of creating the electric field necessary to deliver materials via electrotransfer. The goal was to determine if there was an increase in molecular delivery across the membrane of a human T cell line used as a model system. In a novel dish created for the study, the effects of elevated temperatures (37, 40, 43, and 45°C) during the treatment process were also examined in combination with corona charge application. Results showed that treating cells with corona charge at room temperature (~23°C) caused a statistically significant increase in molecular delivery while maintaining viability. Heat alone did not cause a statistically significant effect on molecular delivery. Combined corona charge treatment and heating resulted in a statistically significant increase on molecular delivery compared to controls that were only heated. Combined corona charge treatment and heating to all temperatures when compared to controls treated at room temperature, showed a statistically significant increase in molecular delivery.
随着癌症基因和免疫疗法的快速发展，需要有效地转染细胞。先前的研究表明，电转移可以为基因传递提供一种非病毒方法。电转移传统上依赖于将直流电脉冲施加到感兴趣的细胞上。本研究探讨了电晕电荷作为传统方法的替代方法，作为通过电转移传递材料所需的电场的产生手段。目的是确定在用作模型系统的人 T 细胞系的膜上是否增加了分子传递。在为该研究创建的新型培养皿中，还研究了在处理过程中升高温度（37、40、43 和 45°C）与施加电晕电荷相结合的影响。结果表明，在室温（约 23°C）下用电晕电荷处理细胞会导致分子传递的统计学显着增加，同时保持细胞活力。单独加热不会对分子传递产生统计学上的显著影响。与仅加热的对照相比，联合电晕电荷处理和加热导致分子传递的统计学显着增加。与在室温下处理的对照相比，联合电晕电荷处理和加热至所有温度均显示出分子传递的统计学显着增加。
