Hamilton Glaucoma Center, The Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California, San Diego, CA.
Department of Ophthalmology and Optometry, Kepler University Hospital, Johannes Kepler University, Linz.
J Glaucoma. 2024 May 1;33(5):355-360. doi: 10.1097/IJG.0000000000002318. Epub 2023 Oct 5.
Trabecular meshwork (TM) pigmentation is not correlated with angiographically determined aqueous humor outflow (AHO) in an ex vivo perfusion model using human eyes.
To evaluate whether segmental TM pigmentation is correlated to segmental AHO in human eyes.
Postmortem human eyes were acquired, and anterior segments were dissected. TM pigmentation was photographed 360-degrees around the eye. The anterior segments were then mounted onto a perfusion apparatus and perfused with Dulbecco's phosphate buffered saline (DPBS) until a stabile baseline outflow facility was achieved. Aqueous angiography (AHO angiography) was performed using fluorescein (2%), and segmental AHO was documented around the limbus using an angiographic camera (Spectralis HRA+OCT). Circumferential and nasal TM pigmentation were compared with respective angiographic outflow imaging using a Pearson correlation analysis.
Segmental TM pigment distribution and segmental AHO were seen. TM pigment was statistically greatest in the inferior quadrant. AHO angiographic outflow was numerically greatest in the nasal quadrant, but this was not statistically significant. No statistically significant correlation was observed (r=-0.083, P =0.06) between segmental TM pigmentation and segmental AHO angiographic signal. Analyzing just the nasal quadrant, a significant weak negative correlation was found (r=-0.296, P =0.001).
Segmental TM pigmentation circumferentially around the eye is not a good proxy for segmental AHO circumferentially around the eye and should not be used to guide trabecular minimally invasive glaucoma surgeries.
在使用人眼的离体灌注模型中，小梁网（TM）色素沉着与血管造影确定的房水流出（AHO）无关。
评估人眼中节段性 TM 色素沉着与节段性 AHO 是否相关。
获取死后人眼，并解剖前节。对 TM 色素沉着进行 360 度拍照。然后将前节安装到灌注装置上，并使用 Dulbecco 的磷酸盐缓冲盐水（DPBS）灌注，直到达到稳定的基线流出能力。使用荧光素（2%）进行房水血管造影（AHO 血管造影），并使用血管造影相机（Spectralis HRA+OCT）在角膜缘周围记录节段性 AHO。使用 Pearson 相关分析比较环周和鼻侧 TM 色素沉着与相应的血管造影流出成像。
观察到节段性 TM 色素分布和节段性 AHO。TM 色素在下方象限统计学上最大。AHO 血管造影流出在鼻象限数值上最大，但无统计学意义。未观察到节段性 TM 色素沉着与节段性 AHO 血管造影信号之间存在统计学显著相关性（r=-0.083，P=0.06）。仅分析鼻象限，发现存在显著的弱负相关（r=-0.296，P=0.001）。
环绕眼睛的节段性 TM 色素沉着不是环绕眼睛的节段性 AHO 的良好替代物，不应用于指导小梁微创青光眼手术。
