Diaz Steven A, McCamant David W
University of Rochester, Department of Chemistry Rochester, New York, New York 14534, United States.
Anal Chem. 2023 Oct 31;95(43):15856-15860. doi: 10.1021/acs.analchem.3c02491. Epub 2023 Oct 18.
By augmentation of the collection optics utilized in transmission-based femtosecond stimulated Raman spectroscopy (FSRS), two novel diffuse reflectance-based femtosecond stimulated Raman spectroscopy (drFSRS) techniques were developed. These techniques were then used to collect the Raman spectra of opaque systems, those being cyclohexane-intercalated poly(tetrafluoroethylene) microbeads and ethanol in 1% intralipid solutions. The resulting drFSRS data from the cyclohexane:PTFE system show significant distortion of the depolarization ratio of the 803 cm cyclohexane peak, indicating a loss of incident pump:probe polarization in a scattering environment. The drFSRS data from the ethanol in 1% intralipid solution demonstrate less signal strength but equal spectral resolution when compared to transmission-based FSRS of the same sample. The results presented in this Technical Note demonstrate the current capabilities of collecting stimulated Raman spectra of opaque systems using drFSRS.
通过增强基于透射的飞秒受激拉曼光谱（FSRS）中使用的收集光学器件，开发了两种新型的基于漫反射的飞秒受激拉曼光谱（drFSRS）技术。然后使用这些技术收集不透明系统的拉曼光谱，这些系统是环己烷插层的聚四氟乙烯微珠和1%脂质乳剂溶液中的乙醇。来自环己烷：聚四氟乙烯系统的drFSRS数据显示，803 cm环己烷峰的去极化率有明显畸变，表明在散射环境中入射泵浦：探测偏振发生了损失。与相同样品的基于透射的FSRS相比，1%脂质乳剂溶液中乙醇的drFSRS数据显示信号强度较低，但光谱分辨率相同。本技术说明中给出的结果展示了使用drFSRS收集不透明系统受激拉曼光谱的当前能力。
