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导入大鼠L6成肌细胞的人类心脏肌动蛋白基因的调控表明其肌生成程序存在缺陷。

Regulation of a human cardiac actin gene introduced into rat L6 myoblasts suggests a defect in their myogenic program.

作者信息

Hickey R, Skoultchi A, Gunning P, Kedes L

出版信息

Mol Cell Biol. 1986 Sep;6(9):3287-90. doi: 10.1128/mcb.6.9.3287-3290.1986.

DOI:10.1128/mcb.6.9.3287-3290.1986
PMID:3785229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367068/
Abstract

The rat myogenic cell line L6E9 induces skeletal but not cardiac alpha-actin mRNA upon fusion to form myotubes. However, when a human cardiac alpha-actin gene was introduced into L6E9 myoblasts, differentiation of the cells led to the accumulation of human gene transcripts in parallel with those derived from the endogenous skeletal alpha-actin gene. This result demonstrates that factors which direct rat myogenesis can regulate a muscle gene from another species and that the L6E9 cells may have a defect in their ability to activate endogenous cardiac actin gene expression.

摘要

大鼠成肌细胞系L6E9在融合形成肌管时可诱导骨骼肌而非心肌α-肌动蛋白mRNA的产生。然而，当将人类心脏α-肌动蛋白基因导入L6E9成肌细胞时，细胞分化导致人类基因转录本的积累，且与内源性骨骼肌α-肌动蛋白基因产生的转录本平行。这一结果表明，指导大鼠肌肉生成的因子可以调节来自另一个物种的肌肉基因，并且L6E9细胞在激活内源性心脏肌动蛋白基因表达的能力上可能存在缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/38e1bbc8bb60/molcellb00093-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/253fe42338ab/molcellb00093-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/56b01364bd7d/molcellb00093-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/38e1bbc8bb60/molcellb00093-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/253fe42338ab/molcellb00093-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/56b01364bd7d/molcellb00093-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b3/367068/38e1bbc8bb60/molcellb00093-0275-a.jpg

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1
Regulation of a human cardiac actin gene introduced into rat L6 myoblasts suggests a defect in their myogenic program.导入大鼠L6成肌细胞的人类心脏肌动蛋白基因的调控表明其肌生成程序存在缺陷。
Mol Cell Biol. 1986 Sep;6(9):3287-90. doi: 10.1128/mcb.6.9.3287-3290.1986.
2
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The levels of vascular smooth as well as skeletal muscle actin mRNAs differ substantially among both myoblast and fibroblast lines with different skeletal myogenic potentials.在具有不同骨骼肌生成潜能的成肌细胞系和成纤维细胞系中，血管平滑肌肌动蛋白和骨骼肌肌动蛋白mRNA的水平存在显著差异。
Cell Mol Biol (Noisy-le-grand). 1992 Dec;38(8):903-13.
6
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Mol Cell Biol. 1984 Aug;4(8):1449-53. doi: 10.1128/mcb.4.8.1449-1453.1984.
7
Induction of endogenous myosin light chain 1 and cardiac alpha-actin expression in L6E9 cells by MyoD1.MyoD1诱导L6E9细胞内源性肌球蛋白轻链1和心肌α-肌动蛋白表达。
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Alpha actin gene exist in an active structural configuration in the proliferating myoblasts as well as in differentiated myotubes of the L6 line.α-肌动蛋白基因在增殖的成肌细胞以及L6细胞系的分化肌管中以活性结构形式存在。
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Cellular localization of muscle and nonmuscle actin mRNAs in chicken primary myogenic cultures: the induction of alpha-skeletal actin mRNA is regulated independently of alpha-cardiac actin gene expression.鸡原代成肌培养物中肌肉和非肌肉肌动蛋白mRNA的细胞定位：α-骨骼肌动蛋白mRNA的诱导独立于α-心肌动蛋白基因表达进行调控。
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本文引用的文献

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The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis.骨骼肌肌动蛋白、肌球蛋白重链和肌球蛋白轻链的合成与肌生成过程中相应mRNA序列积累之间的相关性。
Dev Biol. 1981 Sep;86(2):483-92. doi: 10.1016/0012-1606(81)90206-2.
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Gene switching in myogenesis: differential expression of the chicken actin multigene family.肌肉生成中的基因转换：鸡肌动蛋白多基因家族的差异表达
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Regulated expression of human growth hormone genes in mouse cells.
转染的人心脏肌动蛋白基因在多能性小鼠胚胎癌细胞分化过程中的调控表达。
Mol Cell Biol. 1988 Jan;8(1):406-17. doi: 10.1128/mcb.8.1.406-417.1988.
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A common factor regulates skeletal and cardiac alpha-actin gene transcription in muscle.一个共同因子调节肌肉中骨骼肌和心肌α-肌动蛋白基因的转录。
Mol Cell Biol. 1988 Oct;8(10):4120-33. doi: 10.1128/mcb.8.10.4120-4133.1988.
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Interaction of nuclear proteins with muscle-specific regulatory sequences of the human cardiac alpha-actin promoter.核蛋白与人心脏α-肌动蛋白启动子的肌肉特异性调控序列之间的相互作用。
Mol Cell Biol. 1988 Oct;8(10):4110-9. doi: 10.1128/mcb.8.10.4110-4119.1988.
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Differentiation of cardiac myocytes after mitogen withdrawal exhibits three sequential states of the ventricular growth response.有丝分裂原撤除后心肌细胞的分化表现出心室生长反应的三个连续状态。
J Cell Biol. 1988 Nov;107(5):1911-8. doi: 10.1083/jcb.107.5.1911.
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Transformation and rescue of a flightless Drosophila tropomyosin mutant.一种无法飞行的果蝇原肌球蛋白突变体的转化与拯救
EMBO J. 1987 May;6(5):1375-85. doi: 10.1002/j.1460-2075.1987.tb02378.x.
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Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression.人类α-骨骼肌动蛋白基因的多个5'侧翼区域协同调节肌肉特异性表达。
Mol Cell Biol. 1987 Nov;7(11):4089-99. doi: 10.1128/mcb.7.11.4089-4099.1987.
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The chicken skeletal muscle alpha-actin promoter is tissue specific in transgenic mice.鸡骨骼肌α-肌动蛋白启动子在转基因小鼠中具有组织特异性。
Mol Cell Biol. 1989 Sep;9(9):3785-92. doi: 10.1128/mcb.9.9.3785-3792.1989.
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Sequence and expression of human myosin alkali light chain isoforms.人类肌球蛋白碱性轻链亚型的序列与表达
Mol Cell Biochem. 1989 Jun 1;87(2):119-36. doi: 10.1007/BF00219255.
人生长激素基因在小鼠细胞中的调控表达。
Cell. 1982 Jun;29(2):623-31. doi: 10.1016/0092-8674(82)90178-7.
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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6. doi: 10.1073/pnas.78.4.2072.
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A fetal skeletal muscle actin mRNA in the mouse and its identity with cardiac actin mRNA.小鼠胎儿骨骼肌肌动蛋白信使核糖核酸及其与心肌肌动蛋白信使核糖核酸的一致性
Cell. 1982 Aug;30(1):185-92. doi: 10.1016/0092-8674(82)90024-1.
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alpha-Cardiac actin is the major sarcomeric isoform expressed in embryonic avian skeletal muscle.α-心肌肌动蛋白是在胚胎期禽类骨骼肌中表达的主要肌节亚型。
Science. 1984 Jun 29;224(4656):1436-8. doi: 10.1126/science.6729461.
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Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution.人类肌动蛋白基因中，α-骨骼肌肌动蛋白和α-心肌肌动蛋白为单拷贝基因，而β-细胞骨架肌动蛋白和γ-细胞骨架肌动蛋白为多拷贝基因：3'非翻译区具有同型特异性，但在进化过程中保守。
Mol Cell Biol. 1983 Oct;3(10):1783-91. doi: 10.1128/mcb.3.10.1783-1791.1983.
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Cell type-specific activation of actin genes in the early amphibian embryo.早期两栖动物胚胎中肌动蛋白基因的细胞类型特异性激活。
Nature. 1984;311(5988):716-21. doi: 10.1038/311716a0.
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Expression of the genes coding for the skeletal muscle and cardiac actions in the heart.编码骨骼肌和心脏活动的基因在心脏中的表达。
Nucleic Acids Res. 1984 Jan 25;12(2):1087-100. doi: 10.1093/nar/12.2.1087.
10
Expression of human cardiac actin in mouse L cells: a sarcomeric actin associates with a nonmuscle cytoskeleton.人心脏肌动蛋白在小鼠L细胞中的表达：一种肌节肌动蛋白与非肌肉细胞骨架相关联。
Cell. 1984 Mar;36(3):709-15. doi: 10.1016/0092-8674(84)90351-9.