State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100101, China.
Food Chem Toxicol. 2023 Nov;181:114103. doi: 10.1016/j.fct.2023.114103. Epub 2023 Oct 16.
There are several technical challenges and public issues concerning genome editing applications before they become viable in commercial aquaculture. Recently, we developed a novel strategy to generate all-female (AF) common carp, which exhibited a growth advantage over the control carp, using genetic editing through single gene-targeting manipulation. Here, we found that the body weight of the AF common carp was higher by 22.58% than that of the control common carp. Because the genotype of the AF common carp was cyp17a1+/-;XX, the contents of sex steroids were normally synthesized, as they were comparable to that of the control female carp. To evaluate the food safety of the AF carp, Wistar rats were fed a diet containing control female carp (control, C) or all-female (AF) carp at an incorporation rate of 5, 10 and 20% (w/w) for 90 days. Compared with those fed control carp, the rats fed AF common carp exhibited no significant difference in body weight, food intake, feed conversion ratio, hematology, serum biochemistry, urine test, relative organ weight, gross necropsy, and histopathological examination. This is the first food safety assessment of the farmed fish strain cultured using CRISPR/Cas9, which will further advance the fishery development of genome-edited animals.
在基因组编辑应用在商业水产养殖中可行之前，还存在一些技术挑战和公共问题。最近，我们开发了一种新策略，通过单基因靶向操作的遗传编辑，培育出全雌（AF）鲤鱼，与对照组鲤鱼相比，其生长优势明显。在这里，我们发现 AF 鲤鱼的体重比对照组鲤鱼高 22.58%。因为 AF 鲤鱼的基因型为 cyp17a1+/-;XX，所以其性类固醇的含量正常合成，与对照组雌性鲤鱼相当。为了评估 AF 鲤鱼的食品安全，我们用含有对照组雌性鲤鱼（对照，C）或全雌（AF）鲤鱼的饲料喂养 Wistar 大鼠，其掺入率分别为 5%、10%和 20%（w/w），喂养 90 天。与那些喂食对照组鲤鱼的大鼠相比，喂食 AF 鲤鱼的大鼠在体重、食物摄入量、饲料转化率、血液学、血清生化、尿液检查、相对器官重量、大体尸检和组织病理学检查方面均无显著差异。这是首次对使用 CRISPR/Cas9 培养的养殖鱼类进行的食品安全评估，这将进一步推动基因组编辑动物的渔业发展。
