Li Xuehui, Zou Qingqing, Liu Xuebo, Lou Qiyong, Jin Xia, He Jiangyan, Yin Zhan, Zhai Gang, Duan Ming, Chen Guanghui
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2024 Nov 21;25(23):12517. doi: 10.3390/ijms252312517.
Genome editing has the potential to improve growth and traits of aquatic animals. Assessment of the feeding habits of the genetically modified farmed fish is necessary, as this is closely related to the assessment of their growth performance, which is one of the most important economic traits. Previously, we developed a novel strategy to produce all-female (AF) common carp (+/-;XX genotype) with genome editing, which exhibited a growth advantage compared to the control carp (including control male and female carp). However, the feeding behavior related to the growth performance of wild-type control and AF common carp remains elusive. The results of feeding and swimming behaviors showed that AF common carp exhibited a faster feeding activities and more active swimming activities, which probably enhanced its growth performance. Brain gene expression analysis revealed AF common carp had a significant upregulation of the orexigenic factors gene expression levels in the fed state, which would further promote the growth of AF carp. Here, AF carp exhibited higher growth performance with higher growth hormone () gene expression. This study provided insight into the growth performance, feeding behaviors and appetite regulation of the genetically modified AF carp and the assessment of feeding behaviors in other genetically modified farmed fish.
基因组编辑有潜力改善水生动物的生长和性状。评估转基因养殖鱼类的摄食习性是必要的，因为这与它们生长性能的评估密切相关，而生长性能是最重要的经济性状之一。此前，我们开发了一种利用基因组编辑生产全雌（AF）鲤鱼（+/-;XX基因型）的新策略，与对照鲤鱼（包括对照雄鲤和雌鲤）相比，其表现出生长优势。然而，野生型对照和AF鲤鱼与生长性能相关的摄食行为仍不清楚。摄食和游泳行为的结果表明，AF鲤鱼表现出更快的摄食活动和更活跃的游泳活动，这可能增强了其生长性能。脑基因表达分析显示，AF鲤鱼在进食状态下促食欲因子基因表达水平显著上调，这将进一步促进AF鲤鱼的生长。在此，AF鲤鱼表现出更高的生长性能以及更高的生长激素（）基因表达。本研究为转基因AF鲤鱼的生长性能、摄食行为和食欲调节以及其他转基因养殖鱼类的摄食行为评估提供了见解。
