Zohdy Youssef M, Saini Manpreet, Heit Jeremy, Neill Stewart, Morales-Vargas Bryan, Hoang Kimberly, Pradilla Gustavo, Garzon-Muvdi Tomas
Department of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Department of Pathology, Emory University, Atlanta, Georgia, USA.
World Neurosurg. 2024 Jan;181:e384-e391. doi: 10.1016/j.wneu.2023.10.066. Epub 2023 Oct 16.
Brain tumors display significant inter and intratumoral heterogeneity, impacting disease progression and outcomes. Preserving surgically resected tissue is vital for ensuring accurate research results to enhance understanding of tumor pathophysiology. This study evaluates tissue integrity and viability of tissue resected using 2 surgical devices for tumor resection: a mechanical microdebrider (MD) and an ultrasonic aspirator (UA).
Tumor samples were obtained from patients undergoing surgical resection of primary and secondary intracranial tumors. Cell viability was assessed, and histopathological analysis of Hematoxylin and Eosin -stained tissues was performed. Adherent monolayer and neurospheres cell cultures were established from paired samples. RNA isolation and quantitative polymerase chain reaction of housekeeping genes were conducted to compare genetic integrity.
The cellular viability was comparable between samples obtained using both the MD and the UA, with a mean viability of 75.2% ± 15.6 and 70.7% ± 16.8, respectively (P = 0.318). Histopathological evaluation indicated no discernible differences in cellular integrity between the devices. Cell culture success rates and growth characteristics were similar for both devices. RNA concentration and integrity were well-maintained in both MD and UA samples, with no significant differences (P = 0.855). Quantitative polymerase chain reaction analysis of housekeeping genes showed consistent results across matched tissues from both devices and different tumor pathologies.
Surgical handheld devices provide valuable, high-quality tissue samples for research. Surgeon preference, tumor pathology, and anatomical location dictate device choice. Both MD and UA devices are reliable for obtaining quality tissue specimens, facilitating translational neuro-oncology research.
脑肿瘤表现出显著的肿瘤间和肿瘤内异质性，影响疾病进展和预后。保存手术切除的组织对于确保准确的研究结果以增进对肿瘤病理生理学的理解至关重要。本研究评估了使用两种用于肿瘤切除的手术设备切除的组织的完整性和活力：机械微型清创器（MD）和超声吸引器（UA）。
从接受原发性和继发性颅内肿瘤手术切除的患者中获取肿瘤样本。评估细胞活力，并对苏木精和伊红染色的组织进行组织病理学分析。从配对样本中建立贴壁单层和神经球细胞培养物。进行管家基因的RNA分离和定量聚合酶链反应以比较基因完整性。
使用MD和UA获得的样本之间的细胞活力相当，平均活力分别为75.2%±15.6和70.7%±16.8（P = 0.318）。组织病理学评估表明，两种设备之间在细胞完整性方面没有明显差异。两种设备的细胞培养成功率和生长特征相似。MD和UA样本中的RNA浓度和完整性均得到良好维持，无显著差异（P = 0.855）。管家基因的定量聚合酶链反应分析在来自两种设备和不同肿瘤病理的匹配组织中显示出一致的结果。
手术手持设备为研究提供了有价值的高质量组织样本。外科医生的偏好、肿瘤病理和解剖位置决定了设备的选择。MD和UA设备在获取高质量组织标本方面都是可靠的，有助于转化神经肿瘤学研究。
