Oya Y, Yamamoto K, Tonomura A
Mutat Res. 1986 Dec;172(3):245-53. doi: 10.1016/0165-1218(86)90062-5.
The cytogenetic effect of hydrogen peroxide (H2O2) was investigated in human embryonic fibroblasts. Chromosome-type aberrations were found together with chromatid-type aberrations in metaphase cells harvested 24 h after a single 10-min treatment with 10(-5)-10(-3) M H2O2 in 0.9% NaCl solution. The chromosome-type aberrations were observed to be predominantly dicentrics and deletions. Both types of aberration showed a dose-response relationship to the dose of H2O2 over the range of 10(-5)-1.5 X 10(-4) M H2O2. The intercellular distribution of dicentrics showed a Poisson distribution. Centric and acentric rings and abnormal monocentrics were a minor fraction of the chromosome-type aberrations. The chromatid-type aberrations observed, such as breaks, exchanges and gaps, showed no dose-response relationship. The frequency of isochromatid breaks was higher than that of chromatid breaks and approximately 70% of the isochromatid breaks were found in the centromeric or pericentromeric region. The intercellular distribution of chromatid exchanges showed an over-dispersed distribution. The generation of aberrations by H2O2 was effectively suppressed by catalase and several scavengers of hydroxyl radicals (.OH) such as ethanol, dimethyl sulfoxide (DMSO) and mannitol. This result suggest that .OH plays an essential role in the generation of the chromosome aberrations by H2O2.
在人胚胎成纤维细胞中研究了过氧化氢（H2O2）的细胞遗传学效应。在用0.9%氯化钠溶液中10(-5)-10(-3) M H2O2单次处理10分钟后24小时收获的中期细胞中，发现了染色体型畸变和染色单体型畸变。观察到染色体型畸变主要是双着丝粒和缺失。在10(-5)-1.5×10(-4) M H2O2范围内，两种类型的畸变均显示出与H2O2剂量的剂量反应关系。双着丝粒的细胞间分布呈泊松分布。着丝粒环和无着丝粒环以及异常单着丝粒是染色体型畸变的一小部分。观察到的染色单体型畸变，如断裂、交换和间隙，未显示剂量反应关系。等染色单体断裂的频率高于染色单体断裂，并且约70%的等染色单体断裂发生在着丝粒或着丝粒周围区域。染色单体交换的细胞间分布呈过度分散分布。过氧化氢酶和几种羟基自由基（·OH）清除剂如乙醇、二甲基亚砜（DMSO）和甘露醇有效地抑制了H2O2引起的畸变。该结果表明·OH在H2O2引起的染色体畸变产生中起重要作用。
