School of Environment and Spatial Informatics, China University of Mining and Technology, 1 Daxue Road, Xuzhou, 221116, Jiangsu, China.
State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Environ Sci Pollut Res Int. 2023 Nov;30(53):113932-113947. doi: 10.1007/s11356-023-29949-w. Epub 2023 Oct 19.
This work is designed to counteract the deficiency of targeted research on the PAHs polluted specific soil, especially when the chemicals extremely denatured it. Phenanthrene-contaminated red soil was treated through two-stage process: persulfate oxidation (on dosages of 3.48%, 5.21%, and 6.94%, combined with Fe and β-cyclodextrin, then heated) followed by biodegradation (indigenous bacteria vs. acid-resistant PAHs-degrading microflora (named ADM)) for 90 days. The dosage of oxidant greatly affected the removal efficiencies, which ranged from 46.78 to 85.34% under different treatment. After undergoing oxidation, the soil pH dropped below 3.0 synchronously and retained relatively strong oxidation state. The indigenous bacteria in red soil showed considerable degradation potential that will not vanish upon the sudden change of soil properties, whose average combined removal reached 95.43%, even higher than subgroups of bioaugmentation, but the population structure showed extremely simplex (Proteobacteria as superior occupied proportion of 91.77% after 90-day rehabilitation). The ADM screened from the coking wastewater was dominated by Klebsiella (75.4%) and Pseudomonas (23.6%), whose cooperation with 6.94% persulfate made the residual PHE reduced to less than 50 mg·kg in about 28 days. High-throughput sequencing analysis showed that the microbial community composition of the ADM applied-group was more abundant in the later stage of remediation. ADM inoculation has the advantages of shortening the restoration period and having a positive impact on the soil micro-ecology.
这项工作旨在克服针对特定受多环芳烃污染土壤的靶向研究的不足，尤其是当化学物质使其极度变性时。采用两段法处理菲污染的红壤：过硫酸盐氧化（用量分别为 3.48%、5.21%和 6.94%，结合 Fe 和β-环糊精，然后加热），然后进行生物降解（土著细菌与耐酸多环芳烃降解菌群（命名为 ADM））90 天。氧化剂用量对去除效率有很大影响，不同处理下的去除效率范围为 46.78%至 85.34%。氧化后，土壤 pH 同步降至 3.0 以下，保持较强的氧化状态。红壤中的土著细菌具有相当大的降解潜力，不会因土壤性质的突然变化而消失，其平均综合去除率达到 95.43%，甚至高于生物强化亚组，但种群结构极为简单（经过 90 天修复后，变形菌门占优势，比例为 91.77%）。从焦化废水中筛选出的 ADM 以克雷伯氏菌（75.4%）和假单胞菌（23.6%）为主，它们与 6.94%过硫酸盐的合作使残留的 PHE 在大约 28 天内减少到 50mg·kg 以下。高通量测序分析表明，ADM 应用组的微生物群落组成在修复后期更为丰富。ADM 接种具有缩短修复期和对土壤微生态产生积极影响的优点。
