Liu Bimin, Li Yuxiao, Ren Miao, Li Xiangning
State Key Laboratory of Digital Medical Engineering, School of Biomedical Engineering, Hainan University, Haikou, China.
Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou, China.
Front Cell Neurosci. 2023 Oct 3;17:1259360. doi: 10.3389/fncel.2023.1259360. eCollection 2023.
Understanding the developmental changes that affect neurons is a key step in exploring the assembly and maturation of neural circuits in the brain. For decades, researchers have used a number of labeling techniques to visualize neuronal morphology at different stages of development. However, the efficiency and accuracy of neuronal labeling technologies are limited by the complexity and fragility of neonatal brains. In this review, we illustrate the various labeling techniques utilized for examining the neurogenesis and morphological changes occurring during the early stages of development. We compare the advantages and limitations of each technique from different aspects. Then, we highlight the gaps remaining in our understanding of the structure of neurons in the neonatal mouse brain.
了解影响神经元的发育变化是探索大脑神经回路组装和成熟的关键一步。几十年来，研究人员使用了多种标记技术来观察神经元在不同发育阶段的形态。然而，神经元标记技术的效率和准确性受到新生大脑复杂性和脆弱性的限制。在这篇综述中，我们阐述了用于研究发育早期神经发生和形态变化的各种标记技术。我们从不同方面比较了每种技术的优缺点。然后，我们强调了在理解新生小鼠大脑神经元结构方面仍存在的差距。
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