小鼠子宫内双电穿孔技术用于操纵发育中皮层的两个特定神经元群体

Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex.

作者信息

Zhang Longbo, Getz Stephanie A, Bordey Angelique

机构信息

Departments of Neurosurgery, And National Clinical Research Center of Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Departments of Neurosurgery, And Cellular and Molecular Physiology, School of Medicine, Yale University, New Haven, CT, United States.

出版信息

Front Bioeng Biotechnol. 2022 Jan 12;9:814638. doi: 10.3389/fbioe.2021.814638. eCollection 2021.

DOI:10.3389/fbioe.2021.814638

PMID:35096799

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8790278/

Abstract

Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple neuronal populations within a circuit in a spatially and temporally regulated manner. In addition, this technique allows for morphophysiological investigations into neuronal development and connectivity following cell-specific gene manipulations. Here, we detail the dual electroporation protocol.

摘要

在皮质神经元发育过程中，基因表达的精确调控对于神经元连接的建立和维持以及高阶认知至关重要。子宫内双脉冲电穿孔提供了一种精确有效的工具，能够以空间和时间调控的方式标记和操纵回路中多个神经元群体的基因表达。此外，该技术还允许在细胞特异性基因操作后对神经元发育和连接进行形态生理学研究。在此，我们详细介绍双脉冲电穿孔方案。