微流控环境中贴壁活细胞的全景定量相成像
Panoramic quantitative phase imaging of adherent live cells in a microfluidic environment.
作者信息
Ma Ying, Dai Taiqiang, Lei Yunze, Zhang Linlin, Ma Lin, Liu Min, An Sha, Zheng Juanjuan, Zhuo Kequn, Kong Liang, Gao Peng
机构信息
School of Physics, Xidian University, Xi'an 710071, China.
Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Education, China.
出版信息
Biomed Opt Express. 2023 Sep 13;14(10):5182-5198. doi: 10.1364/BOE.498602. eCollection 2023 Oct 1.
Understanding how cells respond to external stimuli is crucial. However, there are a lack of inspection systems capable of simultaneously stimulating and imaging cells, especially in their natural states. This study presents a novel microfluidic stimulation and observation system equipped with flat-fielding quantitative phase contrast microscopy (FF-QPCM). This system allowed us to track the behavior of organelles in live cells experiencing controlled microfluidic stimulation. Using this innovative imaging platform, we successfully quantified the cellular response to shear stress including directional cellular shrinkage and mitochondrial distribution change in a label-free manner. Additionally, we detected and characterized the cellular response, particularly mitochondrial behavior, under varying fluidic conditions such as temperature and drug induction time. The proposed imaging platform is highly suitable for various microfluidic applications at the organelle level. We advocate that this platform will significantly facilitate life science research in microfluidic environments.
了解细胞如何对外界刺激做出反应至关重要。然而，缺乏能够同时刺激细胞并对其成像的检测系统，尤其是在细胞处于自然状态时。本研究提出了一种配备平场定量相衬显微镜（FF-QPCM）的新型微流控刺激与观察系统。该系统使我们能够追踪经历可控微流控刺激的活细胞中细胞器的行为。利用这个创新的成像平台，我们成功地以无标记方式量化了细胞对剪切应力的反应，包括细胞定向收缩和线粒体分布变化。此外，我们检测并表征了在不同流体条件（如温度和药物诱导时间）下的细胞反应，特别是线粒体行为。所提出的成像平台非常适合细胞器水平的各种微流控应用。我们认为这个平台将极大地促进微流控环境中的生命科学研究。
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