Sil M, Chatterjee D, Sarkar P K
Neurochem Res. 1986 Aug;11(8):1181-91. doi: 10.1007/BF00965946.
Plasma membranes from neuronal perikarya (N), protoplasmic astrocytes (A) and oligodendrocytes (O) of rat brains were analysed with respect to their protein and glycoprotein contents and specificities. SDS-polyacrylamide gel electrophoresis revealed a total number of 23, 17, and 17 major proteins in N, A, and O respectively. Periodate-Schiff's staining showed that approximately 40-60% of these proteins were glycoproteins. The reactivity of these glycoproteins to Con A and WGA was also studied. Selective iodination of whole cells followed by electrophoresis and autoradiography indicated that of the major proteins, only 25% of neuronal and 60% of astroglial and oligodendroglial membrane proteins were exposed outside the cell surface. The overall results suggest that membrane proteins of each of the three cell types studied here have characteristically different internal and external markers differing in size, glycoprotein content, and reactivity of the glycoproteins to lectins.
对大鼠脑神经元胞体（N）、原浆性星形胶质细胞（A）和少突胶质细胞（O）的质膜进行了蛋白质、糖蛋白含量及特异性分析。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，N、A和O中分别有23种、17种和17种主要蛋白质。过碘酸-希夫染色表明，这些蛋白质中约40%-60%为糖蛋白。还研究了这些糖蛋白与刀豆球蛋白A和小麦胚凝集素的反应性。对全细胞进行选择性碘化，然后进行电泳和放射自显影，结果表明，在主要蛋白质中，只有25%的神经元膜蛋白以及60%的星形胶质细胞和少突胶质细胞膜蛋白暴露于细胞表面之外。总体结果表明，此处研究的三种细胞类型的膜蛋白具有特征性的不同内部和外部标记，这些标记在大小、糖蛋白含量以及糖蛋白与凝集素的反应性方面存在差异。
