Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
J Infect Dis. 2024 Jan 12;229(1):262-272. doi: 10.1093/infdis/jiad461.
Periodontitis is an exemplar of dysbiosis associated with the coordinated action of multiple members within the microbial consortium. The polymicrobial synergy and dysbiosis hypothesis proposes a dynamic host-microbiome balance, with certain modulators capable of disrupting eubiosis and driving shifts towards dysbiosis within the community. However, these factors remain to be explored. We established a Porphyromonas gingivalis- or Aggregatibacter actinomycetemcomitans-modified subgingival microbiome model and 16S rRNA sequencing revealed that P. gingivalis and A. actinomycetemcomitans altered the microbiome structure and composition indicated by α and β diversity metrics. P. gingivalis increased the subgingival dysbiosis index (SDI), while A. actinomycetemcomitans resulted in a lower SDI. Furthermore, P. gingivalis-stimulated microbiomes compromised epithelium function and reduced expression of tight junction proteins, whereas A. actinomycetemcomitans yielded mild effects. In conclusion, by inoculating P. gingivalis, we created dysbiotic microcosm biofilms in vitro resembling periodontitis-related subgingival microbiota, exhibiting enhanced dysbiosis and impaired epithelium integrity.
牙周炎是一种与微生物群落中多个成员协同作用相关的失调范例。多微生物协同和失调假说提出了一种动态的宿主-微生物群平衡，某些调节剂能够破坏内共生体并促使群落向失调状态转变。然而，这些因素仍有待探索。我们建立了一种牙龈卟啉单胞菌或伴放线放线杆菌修饰的龈下微生物组模型，16S rRNA 测序显示，牙龈卟啉单胞菌和伴放线放线杆菌改变了微生物组的结构和组成，这表明α和β多样性指标发生了变化。牙龈卟啉单胞菌增加了龈下失调指数（SDI），而伴放线放线杆菌则导致 SDI 降低。此外，牙龈卟啉单胞菌刺激的微生物组破坏了上皮功能并降低了紧密连接蛋白的表达，而伴放线放线杆菌则产生了轻微的影响。总之，通过接种牙龈卟啉单胞菌，我们在体外创造了类似于牙周炎相关龈下微生物群的失调微宇宙生物膜，表现出增强的失调和上皮完整性受损。
