Vion-Dury J, Cupo A, Jarry J, Gobaille S, Mandel P
Neuropeptides. 1986 Oct;8(3):213-9. doi: 10.1016/0143-4179(86)90049-1.
Bilateral Olfactory Bulb Removal (OBR) induced both complex behavioral alterations and a decrease of many neurotransmitter levels. We studied brain levels of the pro-enkephalin related peptides 45 days after OBR. Opioid levels were studied using three different highly specific antisera exhibiting very high affinities in radioimmunoassays in striatum, hypothalamus, hypophysis, brain stem and cortex. Methionine enkephalin levels increase significantly in striatum (42%), hypophysis (94%) and hypothalamus (25%) and non-significantly in the other areas. Leucine-enkephalin levels tended to increase in all dissected structures but a significant increase only occurred in striatum (42%). Octapeptide levels (Methionine-enkephalin-Arg-Gly-Leu) significantly increase in striatum (22%) and decrease in hypophysis (97%) and in brain stem (76%). All these results are partially consistent with the decrease of opiate binding described previously after OBR and suggest a complex imbalance in neurotransmitters after such a sensorial deprivation. It is suggested that the modifications of enkephalinergic neurotransmission might be related to the stressful state induced by OBR.
双侧嗅球切除（OBR）会引发复杂的行为改变以及多种神经递质水平的降低。我们在OBR术后45天研究了脑内前脑啡肽相关肽的水平。使用三种不同的高特异性抗血清研究阿片类物质水平，这些抗血清在纹状体、下丘脑、垂体、脑干和皮质的放射免疫分析中表现出非常高的亲和力。甲硫氨酸脑啡肽水平在纹状体（42%）、垂体（94%）和下丘脑（25%）显著升高，在其他区域无显著变化。亮氨酸脑啡肽水平在所有解剖结构中均有升高趋势，但仅在纹状体显著升高（42%）。八肽水平（甲硫氨酸脑啡肽-精氨酸-甘氨酸-亮氨酸）在纹状体显著升高（22%），在垂体（97%）和脑干（76%）降低。所有这些结果与先前描述的OBR术后阿片类物质结合减少部分一致，并表明这种感觉剥夺后神经递质存在复杂的失衡。提示脑啡肽能神经传递的改变可能与OBR诱导的应激状态有关。
