戊四氮点燃大鼠发作期及发作后大鼠脑内阿片肽含量

Opioid peptides content in the rat brain during the ictal phase and after pentylenetetrazol-kindled rats.

作者信息

Asai M, Matamoros-Trejo G, Talavera E, Cano-Martínez A, Avila M E

机构信息

Laboratorio de Análisis Químicos, Instituto Mexicano de Psiquiatría, Mexico, D.F. Mexico.

出版信息

Comp Biochem Physiol A Physiol. 1995 Sep;112(1):241-5. doi: 10.1016/0300-9629(95)00002-o.

DOI:10.1016/0300-9629(95)00002-o

PMID:7553333

Abstract

We determine the opioid peptide content in the rat brain during the ictal phase and postictal depression after pentylenetetrazol kindling rats. Radioimmunoassays with highly specific antisera risen for Met-enkephalin, Leu-enkephalin and octapeptide, were carried out during the ictal phase, and 15, 30 and 60 min after seizures. We always found an initial IR-Met-enkephalin decrease during the postictal depression content, followed by a reduction in IR-Leu-enkephalin and IR-octapeptide tissular concentration. We suggest a functional and differential release of the opioid peptides, during the postictal depression time-course.

摘要

我们测定了戊四氮点燃大鼠后发作期及发作后抑郁期大鼠脑内阿片肽含量。使用针对甲硫氨酸脑啡肽、亮氨酸脑啡肽和八肽的高特异性抗血清进行放射免疫测定，在发作期以及发作后15、30和60分钟进行。我们始终发现，发作后抑郁期内最初甲硫氨酸脑啡肽免疫反应性降低，随后亮氨酸脑啡肽免疫反应性和八肽组织浓度降低。我们认为在发作后抑郁病程中阿片肽存在功能性差异释放。