原代人鼻腔上皮细胞培养。
Primary Human Nasal Epithelial Cell Culture.
机构信息
The Hospital for Sick Children, Toronto, ON, Canada.
出版信息
Methods Mol Biol. 2024;2725:213-223. doi: 10.1007/978-1-0716-3507-0_13.
PMID:37856027
Abstract
Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.
摘要
原代人鼻腔上皮细胞 (HNEC) 培养物是研究与呼吸道相关的生理学、疾病和治疗的极佳模型。本章详细介绍了从样本采集到在气液界面 (ALI) 条件下培养 HNEC 以生成分化良好、具有功能的气道培养物的方案，包括显示有节律运动的纤毛。
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