Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Mol Plant Microbe Interact. 2024 Jan;37(1):15-24. doi: 10.1094/MPMI-05-23-0058-R. Epub 2024 Jan 29.
Oomycete pathogens secrete numerous crinkling and necrosis proteins (CRNs) to manipulate plant immunity and promote infection. However, the functional mechanism of CRN effectors is still poorly understood. Previous research has shown that the effector PsCRN108 binds to the promoter of s and inhibits their expression, resulting in impaired plant immunity. In this study, we found that in addition to , PsCRN108 also suppressed other () family genes, including . Interestingly, PsCRN108 inhibited the expression of through its promoter, but did not directly bind to its promoter. Instead, PsCRN108 interacted with NbCAMTA2, a negative regulator of plant immunity. NbCAMTA2 was a negative regulator of expression, and PsCRN108 could promote such inhibition activity of NbCAMTA2. Our results elucidated the multiple roles of PsCRN108 in the suppression of plant immunity and revealed a new mechanism by which the CRN effector hijacked transcription factors to affect immunity. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
卵菌病原体分泌大量卷曲和坏死蛋白 (CRN) 来操纵植物免疫并促进感染。然而，CRN 效应子的功能机制仍知之甚少。先前的研究表明，效应子 PsCRN108 结合到 s 的启动子并抑制它们的表达，导致植物免疫受损。在这项研究中，我们发现除了 s 之外，PsCRN108 还抑制其他 () 家族基因，包括 。有趣的是，PsCRN108 通过其启动子抑制 的表达，但并不直接与它的启动子结合。相反，PsCRN108 与 NbCAMTA2 相互作用，NbCAMTA2 是植物免疫的负调节剂。NbCAMTA2 是 表达的负调节剂，而 PsCRN108 可以促进 NbCAMTA2 的这种抑制活性。我们的结果阐明了 PsCRN108 在抑制植物免疫中的多种作用，并揭示了 CRN 效应子劫持转录因子影响免疫的新机制。 [公式：见正文] 版权所有 © 2024 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。
